Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-hydroxy-2-oxoglutarate | substrate inhibition, Ki: 35 mM | Rattus norvegicus | |
beta-Hydroxypyruvate | - |
Rattus norvegicus | |
Bromopyruvate | Ki: 0.018 mM | Rattus norvegicus | |
glyoxylate | Ki: 1.5 mM | Rattus norvegicus | |
Mercaptopyruvate | - |
Rattus norvegicus | |
additional information | double inhibitor studies | Rattus norvegicus | |
NaBr | 18% inhibition at 50 mM, only condensation | Rattus norvegicus | |
NaF | 15% inhibition at 50 mM, only condensation | Rattus norvegicus | |
oxaloacetate | competitive, Ki: 0.22 mM | Rattus norvegicus | |
pyruvate | analogs | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | 11% of total activity | Rattus norvegicus | 5737 | - |
mitochondrion | 89% of total activity | Rattus norvegicus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
rat | - |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
7.3 | - |
- |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
enzyme assay at | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
Bromopyruvate | - |
Rattus norvegicus | |
0.22 | - |
oxaloacetate | competitive | Rattus norvegicus | |
35 | - |
4-hydroxy-2-oxoglutarate | substrate inhibition | Rattus norvegicus |