2-keto-4-Hydroxybutyrate aldolase. Identification as 2-keto-4-hydroxyglutarate aldolase, catalytic properties, and role in mammalian metabolism of L-homoserine

Lane, R.S.; Shapley, A.; Dekker, E.E.; Biochemistry 10, 1353-1364 (1971)

Data extracted from this reference:

Inhibitors

Commentary

Organism

Structure

2-Ketobutyrate

competitive, Ki for 2-keto-4-hydroxybutyrate cleavage: 53 mM, Ki for 2-keto-4-hydroxyglutarate cleavage: 52 mM

Bos taurus

2-Ketoglutarate

competitive, Ki for 2-keto-4-hydroxybutyrate cleavage: 18 mM, Ki for 2-keto-4-hydroxyglutarate cleavage: 18 mM

Bos taurus

glycolaldehyde

competitive, Ki for 2-keto-4-hydroxybutyrate cleavage: 2.7 mM, Ki for 2-keto-4-hydroxyglutarate cleavage: 2.4 mM

Bos taurus

NaBH4

-

Bos taurus

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.1

-

4-hydroxy-alpha-ketoglutarate

-

Bos taurus

3.1

-

2-Keto-4-hydroxybutyrate

-

Bos taurus

Inhibitors

Commentary

Organism

Structure

2-Ketobutyrate

competitive, Ki for 2-keto-4-hydroxybutyrate cleavage: 53 mM, Ki for 2-keto-4-hydroxyglutarate cleavage: 52 mM

Bos taurus

2-Ketoglutarate

competitive, Ki for 2-keto-4-hydroxybutyrate cleavage: 18 mM, Ki for 2-keto-4-hydroxyglutarate cleavage: 18 mM

Bos taurus

glycolaldehyde

competitive, Ki for 2-keto-4-hydroxybutyrate cleavage: 2.7 mM, Ki for 2-keto-4-hydroxyglutarate cleavage: 2.4 mM

Bos taurus

NaBH4

-

Bos taurus

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.1

-

4-hydroxy-alpha-ketoglutarate

-

Bos taurus

3.1

-

2-Keto-4-hydroxybutyrate

-

Bos taurus

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

746867

Schapfl

Extended substrate range of t ...

Escherichia coli

Appl. Microbiol. Biotechnol.

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748294

Mdimegh

HOGA1 gene mutations of prima ...

Homo sapiens

J. Clin. Lab. Anal.

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e22053

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747772

MacDonald

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Homo sapiens

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746641

Huang

Use of a novel microtitration ...

Bos taurus

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721727

Riedel

4-Hydroxy-2-oxoglutarate aldol ...

Homo sapiens

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728378

Williams

The enzyme 4-hydroxy-2-oxoglut ...

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3191-3195

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723542

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Structural and biochemical stu ...

Homo sapiens

PLoS ONE

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10

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Belostotsky

Mutations in DHDPSL are respon ...

Homo sapiens

Am. J. Hum. Genet.

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5255

Dekker

2-keto-4-Hydroxyglutarate aldo ...

Bos taurus

J. Biol. Chem.

267

10507-10514

1992

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5253

Anderson

Rat liver 4-hydroxy-2-ketoglut ...

Rattus norvegicus

Arch. Biochem. Biophys.

236

82-97

1985

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5252

Scholtz

-

Substrates of hydroxyketogluta ...

Rattus norvegicus

Bioorg. Chem.

12

229-234

1984

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5250

Grady

Steady-state kinetics and inhi ...

Bos taurus

Biochemistry

20

2497-2502

1981

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13

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5247

Lane

Sulfhydryl groups in relation ...

Bos taurus

Biochim. Biophys. Acta

481

212-221

1977

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5246

Hansen

Inactivation of bovine liver 2 ...

Bos taurus

Biochemistry

15

2912-2917

1976

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5243

Dekker

2-keto-4-Hydroxyglutarate aldo ...

Bos taurus

Methods Enzymol.

42C

280-285

1975

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3

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2159

Adams

-

Enzymes and intermediates of h ...

Rattus norvegicus

Methods Enzymol.

17B

266-306

1971

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5238

Lane

2-keto-4-Hydroxybutyrate aldol ...

Bos taurus, Mammalia

Biochemistry

10

1353-1364

1971

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2

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5241

Kobes

Variant properties of bovine l ...

Bos taurus

Biochim. Biophys. Acta

251

238-250

1971

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5237

Kuratomi

The metabolism of gamma-hydrox ...

Rattus norvegicus

Biochim. Biophys. Acta

78

617-628

1963

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3

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