2-keto-4-Hydroxybutyrate aldolase. Identification as 2-keto-4-hydroxyglutarate aldolase, catalytic properties, and role in mammalian metabolism of L-homoserine

Lane, R.S.; Shapley, A.; Dekker, E.E.; Biochemistry 10, 1353-1364 (1971)

Data extracted from this reference:

Inhibitors

Commentary

Organism

Structure

2-Ketobutyrate

competitive, Ki for 2-keto-4-hydroxybutyrate cleavage: 53 mM, Ki for 2-keto-4-hydroxyglutarate cleavage: 52 mM

Bos taurus

2-Ketoglutarate

competitive, Ki for 2-keto-4-hydroxybutyrate cleavage: 18 mM, Ki for 2-keto-4-hydroxyglutarate cleavage: 18 mM

Bos taurus

glycolaldehyde

competitive, Ki for 2-keto-4-hydroxybutyrate cleavage: 2.7 mM, Ki for 2-keto-4-hydroxyglutarate cleavage: 2.4 mM

Bos taurus

NaBH4

-

Bos taurus

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.1

-

4-hydroxy-alpha-ketoglutarate

-

Bos taurus

3.1

-

2-Keto-4-hydroxybutyrate

-

Bos taurus

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

2-Keto-4-hydroxybutyrate

Bos taurus

metabolism of L-homoserine

?

-

-

-

4-hydroxy-2-oxoglutarate

Mammalia

catabolism of hydroxyproline, condensation physiologically less important

?

-

-

-

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

2-Keto-4-hydroxybutyrate

D-isomer favoured

5238

Bos taurus

Pyruvate + formaldehyde

-

5238

Bos taurus

-

2-Keto-4-hydroxybutyrate

metabolism of L-homoserine

5238

Bos taurus

?

-

-

-

-

4-Hydroxy-2-oxoglutarate

-

5238

Bos taurus

Pyruvate + glyoxylate

-

5238

Bos taurus

-

4-hydroxy-2-oxoglutarate

catabolism of hydroxyproline, condensation physiologically less important

5238

Mammalia

?

-

-

-

-

additional information

mechanism

5238

Bos taurus

?

-

-

-

-

Pyruvate + formaldehyde

Keq: 4.1 mM

5238

Bos taurus

2-Keto-4-hydroxybutyrate

-

5238

Bos taurus

-

Inhibitors

Commentary

Organism

Structure

2-Ketobutyrate

competitive, Ki for 2-keto-4-hydroxybutyrate cleavage: 53 mM, Ki for 2-keto-4-hydroxyglutarate cleavage: 52 mM

Bos taurus

2-Ketoglutarate

competitive, Ki for 2-keto-4-hydroxybutyrate cleavage: 18 mM, Ki for 2-keto-4-hydroxyglutarate cleavage: 18 mM

Bos taurus

glycolaldehyde

competitive, Ki for 2-keto-4-hydroxybutyrate cleavage: 2.7 mM, Ki for 2-keto-4-hydroxyglutarate cleavage: 2.4 mM

Bos taurus

NaBH4

-

Bos taurus

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.1

-

4-hydroxy-alpha-ketoglutarate

-

Bos taurus

3.1

-

2-Keto-4-hydroxybutyrate

-

Bos taurus

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

2-Keto-4-hydroxybutyrate

Bos taurus

metabolism of L-homoserine

?

-

-

-

4-hydroxy-2-oxoglutarate

Mammalia

catabolism of hydroxyproline, condensation physiologically less important

?

-

-

-

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

2-Keto-4-hydroxybutyrate

D-isomer favoured

5238

Bos taurus

Pyruvate + formaldehyde

-

5238

Bos taurus

-

2-Keto-4-hydroxybutyrate

metabolism of L-homoserine

5238

Bos taurus

?

-

-

-

-

4-Hydroxy-2-oxoglutarate

-

5238

Bos taurus

Pyruvate + glyoxylate

-

5238

Bos taurus

-

4-hydroxy-2-oxoglutarate

catabolism of hydroxyproline, condensation physiologically less important

5238

Mammalia

?

-

-

-

-

additional information

mechanism

5238

Bos taurus

?

-

-

-

-

Pyruvate + formaldehyde

Keq: 4.1 mM

5238

Bos taurus

2-Keto-4-hydroxybutyrate

-

5238

Bos taurus

-

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

746867

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Rattus norvegicus

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5250

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Bos taurus

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1981

2

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13

1

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Lane

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5246

Hansen

Inactivation of bovine liver 2 ...

Bos taurus

Biochemistry

15

2912-2917

1976

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5243

Dekker

2-keto-4-Hydroxyglutarate aldo ...

Bos taurus

Methods Enzymol.

42C

280-285

1975

-

-

-

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1

3

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1

1

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2

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Adams

-

Enzymes and intermediates of h ...

Rattus norvegicus

Methods Enzymol.

17B

266-306

1971

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2

2

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1

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5238

Lane

2-keto-4-Hydroxybutyrate aldol ...

Bos taurus, Mammalia

Biochemistry

10

1353-1364

1971

-

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4

2

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2

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2

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5241

Kobes

Variant properties of bovine l ...

Bos taurus

Biochim. Biophys. Acta

251

238-250

1971

-

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Kuratomi

The metabolism of gamma-hydrox ...

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617-628

1963

-

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