The metabolism of gamma-hydroxyglutamate in rat liver. 1. Enzymic synthesis of gamma-hydroxy-alpha-ketoglutarate from pyruvate and glyoxylate

Kuratomi, K.; Fukunaga, K.; Biochim. Biophys. Acta 78, 617-628 (1963)

Data extracted from this reference:

Inhibitors

Commentary

Organism

Structure

arsenite

-

Rattus norvegicus

Cu2+

93% inhibition at 2 mM

Rattus norvegicus

cysteine

65% inhibition at 10 mM

Rattus norvegicus

Hg2+

complete inhibition at 2 mM

Rattus norvegicus

iodoacetate

10% inhibition at 10 mM

Rattus norvegicus

N-ethylmaleimide

64% inhibition at 10 mM

Rattus norvegicus

p-chloromercuribenzoate

complete inhibition at 10 mM

Rattus norvegicus

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.43

-

glyoxylate

-

Rattus norvegicus

1.33

-

4-hydroxy-alpha-ketoglutarate

-

Rattus norvegicus

10

-

pyruvate

-

Rattus norvegicus

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

4-Hydroxy-2-oxoglutarate

-

5237

Rattus norvegicus

Pyruvate + glyoxylate

-

5237

Rattus norvegicus

-

Glyoxylate + pyruvate

specific, codensation favoured, Keq: 0.73 mM

5237

Rattus norvegicus

4-Hydroxy-2-ketoglutarate

-

5237

Rattus norvegicus

-

additional information

specific for glyoxylate and pyruvate

5237

Rattus norvegicus

?

-

-

-

-

additional information

equilibrium favours condensation, Keq: 0.73 1/mM

5237

Rattus norvegicus

?

-

-

-

-

Inhibitors

Commentary

Organism

Structure

arsenite

-

Rattus norvegicus

Cu2+

93% inhibition at 2 mM

Rattus norvegicus

cysteine

65% inhibition at 10 mM

Rattus norvegicus

Hg2+

complete inhibition at 2 mM

Rattus norvegicus

iodoacetate

10% inhibition at 10 mM

Rattus norvegicus

N-ethylmaleimide

64% inhibition at 10 mM

Rattus norvegicus

p-chloromercuribenzoate

complete inhibition at 10 mM

Rattus norvegicus

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.43

-

glyoxylate

-

Rattus norvegicus

1.33

-

4-hydroxy-alpha-ketoglutarate

-

Rattus norvegicus

10

-

pyruvate

-

Rattus norvegicus

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

4-Hydroxy-2-oxoglutarate

-

5237

Rattus norvegicus

Pyruvate + glyoxylate

-

5237

Rattus norvegicus

-

Glyoxylate + pyruvate

specific, codensation favoured, Keq: 0.73 mM

5237

Rattus norvegicus

4-Hydroxy-2-ketoglutarate

-

5237

Rattus norvegicus

-

additional information

specific for glyoxylate and pyruvate

5237

Rattus norvegicus

?

-

-

-

-

additional information

equilibrium favours condensation, Keq: 0.73 1/mM

5237

Rattus norvegicus

?

-

-

-

-

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

746867

Schapfl

Extended substrate range of t ...

Escherichia coli

Appl. Microbiol. Biotechnol.

102

8359-8372

2018

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748294

Mdimegh

HOGA1 gene mutations of prima ...

Homo sapiens

J. Clin. Lab. Anal.

31

e22053

2017

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1

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747772

MacDonald

Cellular degradation of 4-hyd ...

Homo sapiens

FEBS Lett.

590

1467-1476

2016

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1

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746641

Huang

Use of a novel microtitration ...

Bos taurus

Acta Crystallogr. Sect. F

70

1546-1549

2014

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1

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721727

Riedel

4-Hydroxy-2-oxoglutarate aldol ...

Homo sapiens

Biochim. Biophys. Acta

1822

1544-1552

2012

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1

1

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9

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9

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728378

Williams

The enzyme 4-hydroxy-2-oxoglut ...

Homo sapiens

Nephrol. Dial. Transplant.

27

3191-3195

2012

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723542

Riedel

Structural and biochemical stu ...

Homo sapiens

PLoS ONE

6

e26021

2011

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1

1

11

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10

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2

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10

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10

10

713711

Belostotsky

Mutations in DHDPSL are respon ...

Homo sapiens

Am. J. Hum. Genet.

87

392-399

2010

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1

1

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5255

Dekker

2-keto-4-Hydroxyglutarate aldo ...

Bos taurus

J. Biol. Chem.

267

10507-10514

1992

1

-

-

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-

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7

2

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1

6

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4

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1

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5

1

3

5

1

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1

2

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1

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7

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2

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1

6

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1

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5

1

3

5

1

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1

2

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-

-

5253

Anderson

Rat liver 4-hydroxy-2-ketoglut ...

Rattus norvegicus

Arch. Biochem. Biophys.

236

82-97

1985

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10

1

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5252

Scholtz

-

Substrates of hydroxyketogluta ...

Rattus norvegicus

Bioorg. Chem.

12

229-234

1984

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1

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-

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9

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1

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5250

Grady

Steady-state kinetics and inhi ...

Bos taurus

Biochemistry

20

2497-2502

1981

2

-

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-

-

13

1

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1

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3

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5247

Lane

Sulfhydryl groups in relation ...

Bos taurus

Biochim. Biophys. Acta

481

212-221

1977

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2

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2

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1

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2

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1

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5246

Hansen

Inactivation of bovine liver 2 ...

Bos taurus

Biochemistry

15

2912-2917

1976

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-

-

-

1

1

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1

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3

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1

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5243

Dekker

2-keto-4-Hydroxyglutarate aldo ...

Bos taurus

Methods Enzymol.

42C

280-285

1975

-

-

-

-

-

-

1

3

-

1

1

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2

-

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1

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2

1

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2

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2159

Adams

-

Enzymes and intermediates of h ...

Rattus norvegicus

Methods Enzymol.

17B

266-306

1971

-

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-

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-

-

2

2

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1

1

1

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1

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4

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4

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-

5238

Lane

2-keto-4-Hydroxybutyrate aldol ...

Bos taurus, Mammalia

Biochemistry

10

1353-1364

1971

-

-

-

-

-

-

4

2

-

2

-

2

-

2

-

-

1

-

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1

2

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6

-

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1

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1

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2

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4

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2

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6

-

1

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1

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2

2

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-

-

-

-

5241

Kobes

Variant properties of bovine l ...

Bos taurus

Biochim. Biophys. Acta

251

238-250

1971

-

-

-

-

-

-

1

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1

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2

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2

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7

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7

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5237

Kuratomi

The metabolism of gamma-hydrox ...

Rattus norvegicus

Biochim. Biophys. Acta

78

617-628

1963

-

-

-

-

-

-

7

3

1

1

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1

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1

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3

1

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4

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1

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7

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