BRENDA - Enzyme Database
show all sequences of 4.1.3.16

2-keto-4-Hydroxybutyrate aldolase. Identification as 2-keto-4-hydroxyglutarate aldolase, catalytic properties, and role in mammalian metabolism of L-homoserine

Lane, R.S.; Shapley, A.; Dekker, E.E.; Biochemistry 10, 1353-1364 (1971)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2-Ketobutyrate
competitive, Ki for 2-keto-4-hydroxybutyrate cleavage: 53 mM, Ki for 2-keto-4-hydroxyglutarate cleavage: 52 mM
Bos taurus
2-Ketoglutarate
competitive, Ki for 2-keto-4-hydroxybutyrate cleavage: 18 mM, Ki for 2-keto-4-hydroxyglutarate cleavage: 18 mM
Bos taurus
glycolaldehyde
competitive, Ki for 2-keto-4-hydroxybutyrate cleavage: 2.7 mM, Ki for 2-keto-4-hydroxyglutarate cleavage: 2.4 mM
Bos taurus
NaBH4
-
Bos taurus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
4-hydroxy-alpha-ketoglutarate
-
Bos taurus
3.1
-
2-Keto-4-hydroxybutyrate
-
Bos taurus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
no requirement
Bos taurus
additional information
no requirement
Mammalia
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2-Keto-4-hydroxybutyrate
Bos taurus
metabolism of L-homoserine
?
-
-
-
4-hydroxy-2-oxoglutarate
Mammalia
catabolism of hydroxyproline, condensation physiologically less important
?
-
-
-
Organism
Organism
UniProt
Commentary
Textmining
Bos taurus
-
bovine
-
Mammalia
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Bos taurus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.53
-
2-keto-4-hydroxybutyrate
Bos taurus
1.8
-
2-keto-4-hydroxyglutarate
Bos taurus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2-Keto-4-hydroxybutyrate
D-isomer favoured
5238
Bos taurus
Pyruvate + formaldehyde
-
5238
Bos taurus
-
2-Keto-4-hydroxybutyrate
metabolism of L-homoserine
5238
Bos taurus
?
-
-
-
-
4-Hydroxy-2-oxoglutarate
-
5238
Bos taurus
Pyruvate + glyoxylate
-
5238
Bos taurus
-
4-hydroxy-2-oxoglutarate
catabolism of hydroxyproline, condensation physiologically less important
5238
Mammalia
?
-
-
-
-
additional information
mechanism
5238
Bos taurus
?
-
-
-
-
Pyruvate + formaldehyde
Keq: 4.1 mM
5238
Bos taurus
2-Keto-4-hydroxybutyrate
-
5238
Bos taurus
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
enzyme assay at
Bos taurus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
59
-
constant decay of 0.011 1/min, slow inactivation
Bos taurus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.1
-
2-keto-4-hydroxybutyrate cleavage
Bos taurus
8.8
-
2-keto-4-hydroxyglutarate cleavage
Bos taurus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
9.5
2-keto-4-hydroxybutyrate cleavage
Bos taurus
7
9
2-keto-4-hydroxyglutarate cleavage
Bos taurus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-Ketobutyrate
competitive, Ki for 2-keto-4-hydroxybutyrate cleavage: 53 mM, Ki for 2-keto-4-hydroxyglutarate cleavage: 52 mM
Bos taurus
2-Ketoglutarate
competitive, Ki for 2-keto-4-hydroxybutyrate cleavage: 18 mM, Ki for 2-keto-4-hydroxyglutarate cleavage: 18 mM
Bos taurus
glycolaldehyde
competitive, Ki for 2-keto-4-hydroxybutyrate cleavage: 2.7 mM, Ki for 2-keto-4-hydroxyglutarate cleavage: 2.4 mM
Bos taurus
NaBH4
-
Bos taurus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
4-hydroxy-alpha-ketoglutarate
-
Bos taurus
3.1
-
2-Keto-4-hydroxybutyrate
-
Bos taurus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
no requirement
Bos taurus
additional information
no requirement
Mammalia
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2-Keto-4-hydroxybutyrate
Bos taurus
metabolism of L-homoserine
?
-
-
-
4-hydroxy-2-oxoglutarate
Mammalia
catabolism of hydroxyproline, condensation physiologically less important
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Bos taurus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.53
-
2-keto-4-hydroxybutyrate
Bos taurus
1.8
-
2-keto-4-hydroxyglutarate
Bos taurus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2-Keto-4-hydroxybutyrate
D-isomer favoured
5238
Bos taurus
Pyruvate + formaldehyde
-
5238
Bos taurus
-
2-Keto-4-hydroxybutyrate
metabolism of L-homoserine
5238
Bos taurus
?
-
-
-
-
4-Hydroxy-2-oxoglutarate
-
5238
Bos taurus
Pyruvate + glyoxylate
-
5238
Bos taurus
-
4-hydroxy-2-oxoglutarate
catabolism of hydroxyproline, condensation physiologically less important
5238
Mammalia
?
-
-
-
-
additional information
mechanism
5238
Bos taurus
?
-
-
-
-
Pyruvate + formaldehyde
Keq: 4.1 mM
5238
Bos taurus
2-Keto-4-hydroxybutyrate
-
5238
Bos taurus
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
enzyme assay at
Bos taurus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
59
-
constant decay of 0.011 1/min, slow inactivation
Bos taurus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.1
-
2-keto-4-hydroxybutyrate cleavage
Bos taurus
8.8
-
2-keto-4-hydroxyglutarate cleavage
Bos taurus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
9.5
2-keto-4-hydroxybutyrate cleavage
Bos taurus
7
9
2-keto-4-hydroxyglutarate cleavage
Bos taurus
Other publictions for EC 4.1.3.16
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746867
Schapfl
Extended substrate range of t ...
Escherichia coli
Appl. Microbiol. Biotechnol.
102
8359-8372
2018
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1
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1
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1
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1
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1
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1
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1
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1
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-
748294
Mdimegh
HOGA1 gene mutations of prima ...
Homo sapiens
J. Clin. Lab. Anal.
31
e22053
2017
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-
-
-
2
-
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1
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2
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1
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1
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2
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1
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1
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-
-
-
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1
1
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747772
MacDonald
Cellular degradation of 4-hyd ...
Homo sapiens
FEBS Lett.
590
1467-1476
2016
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-
1
-
-
-
-
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1
1
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1
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1
1
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-
1
1
-
-
1
-
-
-
-
-
-
1
1
-
-
-
746641
Huang
Use of a novel microtitration ...
Bos taurus
Acta Crystallogr. Sect. F
70
1546-1549
2014
-
-
-
1
-
-
-
-
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1
1
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4
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1
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1
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1
1
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1
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1
1
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-
-
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-
-
-
-
-
-
-
721727
Riedel
4-Hydroxy-2-oxoglutarate aldol ...
Homo sapiens
Biochim. Biophys. Acta
1822
1544-1552
2012
-
1
1
-
9
-
-
-
-
-
2
-
-
4
-
-
-
-
-
-
1
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1
1
1
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1
1
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9
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2
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1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
728378
Williams
The enzyme 4-hydroxy-2-oxoglut ...
Homo sapiens
Nephrol. Dial. Transplant.
27
3191-3195
2012
-
-
1
-
1
-
-
-
-
-
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1
-
2
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1
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1
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1
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1
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1
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1
1
-
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-
723542
Riedel
Structural and biochemical stu ...
Homo sapiens
PLoS ONE
6
e26021
2011
-
-
1
1
11
-
-
10
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2
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2
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2
2
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10
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1
-
1
11
-
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-
-
10
-
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2
-
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-
2
2
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-
-
10
-
-
-
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-
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-
10
10
713711
Belostotsky
Mutations in DHDPSL are respon ...
Homo sapiens
Am. J. Hum. Genet.
87
392-399
2010
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1
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2
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1
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-
-
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1
1
-
-
-
5255
Dekker
2-keto-4-Hydroxyglutarate aldo ...
Bos taurus
J. Biol. Chem.
267
10507-10514
1992
1
-
-
-
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-
7
2
-
1
6
-
-
4
-
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1
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-
5
1
3
5
1
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1
2
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1
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7
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2
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1
6
-
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1
-
5
1
3
5
1
-
-
-
-
1
2
-
-
-
-
-
-
-
-
5253
Anderson
Rat liver 4-hydroxy-2-ketoglut ...
Rattus norvegicus
Arch. Biochem. Biophys.
236
82-97
1985
-
-
-
-
-
-
10
1
2
1
1
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2
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1
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2
2
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1
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3
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10
3
1
2
1
1
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1
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2
2
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-
1
-
-
-
-
-
-
-
-
-
-
-
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-
5252
Scholtz
-
Substrates of hydroxyketogluta ...
Rattus norvegicus
Bioorg. Chem.
12
229-234
1984
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1
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-
-
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9
-
1
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1
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9
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1
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1
2
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3
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5250
Grady
Steady-state kinetics and inhi ...
Bos taurus
Biochemistry
20
2497-2502
1981
2
-
-
-
-
-
13
1
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1
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3
-
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2
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1
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2
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13
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1
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1
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2
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1
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5247
Lane
Sulfhydryl groups in relation ...
Bos taurus
Biochim. Biophys. Acta
481
212-221
1977
-
-
-
-
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2
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1
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2
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2
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1
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2
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1
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5246
Hansen
Inactivation of bovine liver 2 ...
Bos taurus
Biochemistry
15
2912-2917
1976
-
-
-
-
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1
1
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1
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3
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1
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5243
Dekker
2-keto-4-Hydroxyglutarate aldo ...
Bos taurus
Methods Enzymol.
42C
280-285
1975
-
-
-
-
-
-
1
3
-
1
1
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2
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1
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2
1
1
2
-
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1
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1
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3
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1
1
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1
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2
1
1
2
-
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1
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-
2159
Adams
-
Enzymes and intermediates of h ...
Rattus norvegicus
Methods Enzymol.
17B
266-306
1971
-
-
-
-
-
-
2
2
-
1
1
1
-
1
-
-
1
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1
1
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4
-
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1
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2
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2
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1
1
1
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1
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1
1
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4
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1
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-
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-
-
5238
Lane
2-keto-4-Hydroxybutyrate aldol ...
Bos taurus, Mammalia
Biochemistry
10
1353-1364
1971
-
-
-
-
-
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4
2
-
2
-
2
-
2
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1
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1
2
-
6
-
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1
-
1
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2
2
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-
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4
-
2
-
2
-
2
-
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1
-
1
2
-
6
-
1
-
1
-
2
2
-
-
-
-
-
-
-
-
5241
Kobes
Variant properties of bovine l ...
Bos taurus
Biochim. Biophys. Acta
251
238-250
1971
-
-
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5237
Kuratomi
The metabolism of gamma-hydrox ...
Rattus norvegicus
Biochim. Biophys. Acta
78
617-628
1963
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