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Literature summary for 4.1.3.1 extracted from
- Activity and functional properties of the isocitrate lyase in the cyanobacterium Cyanothece sp. PCC 7424 (2017), Microbiology, 163, 731-744 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Gloeothece citriformis PCC 7424 |
General Stability
| General Stability | Organism |
|---|---|
| freezing and thawing reduce the enzyme activity significantly | Gloeothece citriformis PCC 7424 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phosphoenolpyruvate | 90% inhibition at 2 mM | Gloeothece citriformis PCC 7424 |  |
| succinate | 90% inhibition at 5 mM | Gloeothece citriformis PCC 7424 | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.06 | - |
DL-isocitrate | at pH 7.4 and 25°C | Gloeothece citriformis PCC 7424 | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required, in the absence of Mg2+, no enzymatic activity is measured | Gloeothece citriformis PCC 7424 | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate | Gloeothece citriformis PCC 7424 | - |
succinate + glyoxylate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gloeothece citriformis PCC 7424 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| glutathione agarose column chromatography | Gloeothece citriformis PCC 7424 |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 50 mM Tris/HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 1 mM dithiothreitol, few days, the enzyme remains stable | Gloeothece citriformis PCC 7424 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DL-isocitrate | - |
Gloeothece citriformis PCC 7424 | succinate + glyoxylate | - |
? | |
| isocitrate | - |
Gloeothece citriformis PCC 7424 | succinate + glyoxylate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 54000, SDS-PAGE | Gloeothece citriformis PCC 7424 |
| ? | x * 54500, calculated from amino acid sequence | Gloeothece citriformis PCC 7424 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ICL | - |
Gloeothece citriformis PCC 7424 |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Gloeothece citriformis PCC 7424 | enzyme activity is induced under alternating day/night cycles | up |
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