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Literature summary for 4.1.3.1 extracted from
- Operation of glyoxylate cycle in halophilic archaea: presence of malate synthase and isocitrate lyase in Haloferax volcanii (1998), FEBS Lett., 434, 13-16.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | - |
Haloferax volcanii |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | reversible loss of activity in isocitrate lyase from Haloferax volcanii when the enzyme is out of extract without a thiol, such as dithiothreitol | Haloferax volcanii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.16 | - |
D-isocitrate | pH 7.0, 40°C | Haloferax volcanii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
3 * 47000, SDS-PAGE | Haloferax volcanii |
| 140000 | - |
gel filtration | Haloferax volcanii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate | Haloferax volcanii | key enzyme of the glyoxylate cycle | succinate + glyoxylate | - |
? | |
| isocitrate | Haloferax volcanii DSM 3757 | key enzyme of the glyoxylate cycle | succinate + glyoxylate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Haloferax volcanii | - |
- |
- |
| Haloferax volcanii DSM 3757 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Haloferax volcanii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:acetate-grown cell | no activity can be detected when lactate is the carbon source, but high activity is measured when acetate is | Haloferax volcanii | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-isocitrate | - |
Haloferax volcanii | succinate + glyoxylate | - |
? | |
| D-isocitrate | - |
Haloferax volcanii DSM 3757 | succinate + glyoxylate | - |
? | |
| isocitrate | key enzyme of the glyoxylate cycle | Haloferax volcanii | succinate + glyoxylate | - |
? | |
| isocitrate | key enzyme of the glyoxylate cycle | Haloferax volcanii DSM 3757 | succinate + glyoxylate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 47000, SDS-PAGE | Haloferax volcanii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
assay at | Haloferax volcanii |
| 55 | - |
- |
Haloferax volcanii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 60 | 40°C: 50% of maximal activity, 60°C: about 40% of maximal activity | Haloferax volcanii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Haloferax volcanii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.5 | pH 6.5: about 70% of maximal activity, pH 7.5: about 55% of maximal activity | Haloferax volcanii |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | key enzyme of the glyoxylate cycle | Haloferax volcanii |
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