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Literature summary for 4.1.3.1 extracted from
- Cloning and expression of the isocitrate lyase gene from a nitrogen-fixing bacterium, Azotobacter vinelandii, and functional analysis of the enzyme by site-directed mutagenesis (2012), Biosci. Biotechnol. Biochem., 76, 78-83.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene icl, expression of N-terminally His6-tagged enzyme in Escherichia coli strains XL-1 Blue, KM22 (a DELTArecBCD mutant), and ACA421, the latter is defective in isocitrate lyase | Azotobacter vinelandii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate | Azotobacter vinelandii | - |
succinate + glyoxylate | - |
? |  |
| isocitrate | Azotobacter vinelandii IAM1078 | - |
succinate + glyoxylate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Azotobacter vinelandii | A8C781 | gene icl | - |
| Azotobacter vinelandii IAM1078 | A8C781 | gene icl | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant N-terminally His6-tagged enzyme from Escherichia coli by nickel affinity chromatography | Azotobacter vinelandii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:acetate-grown cell | cell growth is similar in glucose- or sodium acetate-medium, but the enzyme activity is 43fold higher in acetate-grown cells | Azotobacter vinelandii | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate | - |
Azotobacter vinelandii | succinate + glyoxylate | - |
? | |
| isocitrate | - |
Azotobacter vinelandii IAM1078 | succinate + glyoxylate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ICL | - |
Azotobacter vinelandii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Azotobacter vinelandii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the amino acid residue at position 504 is involved in thermostability of the ICL enzymes | Azotobacter vinelandii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
assay at | Azotobacter vinelandii |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Azotobacter vinelandii | enzyme expression is induced by acetate | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the isocitrate lyase ICL subfamily 3, one of two eubacterial groups, it has a substitution of M504I compared to the cold-adapted ICLs, rendering is less thermostable, phylogenetic analysis, overview | Azotobacter vinelandii |
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