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Literature summary for 4.1.3.1 extracted from

  • Sanchez, N.S.; Arreguin, R.; Calahorra, M.; Pena, A.
    Effects of salts on aerobic metabolism of Debaryomyces hansenii (2008), FEMS Yeast Res., 8, 1303-1312.
    View publication on PubMed

Application

Application Comment Organism
molecular biology cell growth in presence of high salt concentrations (1 M NaCl or KCl) leads to increase in enzyme activity consisting with higher levels of succinate and decreased levels of isocitrate Debaryomyces hansenii

Organism

Organism UniProt Comment Textmining
Debaryomyces hansenii
-
Y7426
-
Debaryomyces hansenii Y7426
-
Y7426
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0029
-
in cell-free extracts after cell growth in YPD medium Debaryomyces hansenii
0.0048
-
in cell-free extracts after cell growth in YPD medium containing 1 M NaCl Debaryomyces hansenii
0.005
-
in cell-free extracts after cell growth in YPD medium containing 1 M KCl Debaryomyces hansenii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate pH 7.3, cell-free extracts, in presence of MgCl2 and 2,4-dinitrophenylhydrazine Debaryomyces hansenii succinate + glyoxylate analysis based on absorbance at 450 nm ?
isocitrate pH 7.3, cell-free extracts, in presence of MgCl2 and 2,4-dinitrophenylhydrazine Debaryomyces hansenii Y7426 succinate + glyoxylate analysis based on absorbance at 450 nm ?

Synonyms

Synonyms Comment Organism
citrate lyase
-
Debaryomyces hansenii
ICL
-
Debaryomyces hansenii