Literature summary for 4.1.3.1 extracted from

Rua, J.; Soler, J.; Busto, F.; de Arriaga, D.
Specific and reversible inactivation of Phycomyces blakesleeanus isocitrate lyase by ascorbate-iron: role of two redox-active cysteines (2002), Fungal Genet. Biol., 35, 223-234.

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Inhibitors

Inhibitors	Comment	Organism	Structure
ascorbate	enzyme is inhibited by an ascorbate plus Fe2+ system under aerobic conditions,16% residual activity at 2 mM ascorbate/0.02 mM Fe2+. Inactivation requires hydrogen peroxide, and can be prevented by catalase, EDTA, Mg2+, isocitrate, 20 mM glutathione, 20 mM dithiothreitol or 40 mM L-cysteine	Phycomyces blakesleeanus
Fe2+	enzyme is inhibited by an ascorbate plus Fe2+ system under aerobic conditions,16% residual activity at 2 mM ascorbate/0.02 mM Fe2+. Inactivation requires hydrogen peroxide, and can be prevented by catalase, EDTA, Mg2+, isocitrate, 20 mM glutathione, 20 mM dithiothreitol or 40 mM L-cysteine	Phycomyces blakesleeanus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
isocitrate	Phycomyces blakesleeanus	-	succinate + glyoxylate	-	?

Organism

Organism	UniProt	Comment	Textmining
Phycomyces blakesleeanus	-	-	-

Storage Stability

Storage Stability	Organism
removal of dithiothreitol during storage leads to spontaneous and slow inactivation	Phycomyces blakesleeanus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
isocitrate	-	Phycomyces blakesleeanus	succinate + glyoxylate	-	?

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Release 2023.1 (January 2023)