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Literature summary for 4.1.3.1 extracted from

  • Chell, R.M.; Sundaram, T.K.; Wilkinson, A.E.
    Isolation and characterization of isocitrate lyase from a thermophilic Bacillus sp. (1978), Biochem. J., 173, 165-177.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.3.1

Inhibitors

Inhibitors Comment Organism Structure
Maleate condensation reaction Bacillus sp. (in: Bacteria)
malonate condensation reaction Bacillus sp. (in: Bacteria)
methylenesuccinate condensation reaction Bacillus sp. (in: Bacteria)
p-chloromercuribenzoate 100% inhibition at 0.02 mM, reversible by 2-mercaptoethanol Bacillus sp. (in: Bacteria)
phosphoenolpyruvate
-
 Bacillus sp. (in: Bacteria)
succinate
-
 Bacillus sp. (in: Bacteria)
Urea partial inactivation Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 threo-Ds-isocitrate 25 mM imidazole buffer, pH 6.8 Bacillus sp. (in: Bacteria)
0.2
-
 threo-Ds-isocitrate 0.4 M KCl, 25 mM imidazole buffer, pH 6.8 Bacillus sp. (in: Bacteria)

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ absolute requirement Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 4 * 48000, SDS-PAGE Bacillus sp. (in: Bacteria)
180000
-
 sedimentation equilibrium centrifugation, gel filtration Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isocitrate Bacillus sp. (in: Bacteria) glyoxylate cycle ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
 thermophilic
-

Purification (Commentary)

Purification (Comment) Organism
thermophilic Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.9
-
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3S)-isocitrate
-
 Bacillus sp. (in: Bacteria) succinate + glyoxylate
-
 ?
isocitrate
-
 Bacillus sp. (in: Bacteria) succinate + glyoxylate
-
 r
isocitrate glyoxylate cycle Bacillus sp. (in: Bacteria) ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 48000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 KCl, MgCl2, CaCl2 stabilize Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
24
-
 threo-Ds-isocitrate
-
 Bacillus sp. (in: Bacteria)