Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Maleate | condensation reaction | Bacillus sp. (in: Bacteria) | |
malonate | condensation reaction | Bacillus sp. (in: Bacteria) | |
methylenesuccinate | condensation reaction | Bacillus sp. (in: Bacteria) | |
p-chloromercuribenzoate | 100% inhibition at 0.02 mM, reversible by 2-mercaptoethanol | Bacillus sp. (in: Bacteria) | |
phosphoenolpyruvate | - |
Bacillus sp. (in: Bacteria) | |
succinate | - |
Bacillus sp. (in: Bacteria) | |
Urea | partial inactivation | Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
threo-Ds-isocitrate | 25 mM imidazole buffer, pH 6.8 | Bacillus sp. (in: Bacteria) | |
0.2 | - |
threo-Ds-isocitrate | 0.4 M KCl, 25 mM imidazole buffer, pH 6.8 | Bacillus sp. (in: Bacteria) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | absolute requirement | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
4 * 48000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
180000 | - |
sedimentation equilibrium centrifugation, gel filtration | Bacillus sp. (in: Bacteria) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate | Bacillus sp. (in: Bacteria) | glyoxylate cycle | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
thermophilic | - |
Purification (Comment) | Organism |
---|---|
thermophilic | Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.9 | - |
- |
Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-isocitrate | - |
Bacillus sp. (in: Bacteria) | succinate + glyoxylate | - |
? | |
isocitrate | - |
Bacillus sp. (in: Bacteria) | succinate + glyoxylate | - |
r | |
isocitrate | glyoxylate cycle | Bacillus sp. (in: Bacteria) | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 48000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
KCl, MgCl2, CaCl2 stabilize | Bacillus sp. (in: Bacteria) |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
24 | - |
threo-Ds-isocitrate | - |
Bacillus sp. (in: Bacteria) |