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Literature summary for 4.1.2.9 extracted from

  • Henard, C.A.; Smith, H.K.; Guarnieri, M.T.
    Phosphoketolase overexpression increases biomass and lipid yield from methane in an obligate methanotrophic biocatalyst (2017), Metab. Eng., 41, 152-158 .
    View publication on PubMed
Search for more literature for 4.1.2.9

Application

Application Comment Organism
biofuel production overexpression of the PktB isoform leads to a 2fold increase in intracellular acetyl-CoA concentration, and a 2.6fold yield enhancement from methane to microbial biomass and lipids compared to wild-type, increasing the potential for methanotroph lipid-based fuel production Methylotuvimicrobium buryatense
synthesis given that acetyl-CoA is a key intermediate in several biosynthetic pathways, phosphoketolase overexpression offers a viable strategy to enhance the economics of an array of biological methane conversion processes Methylotuvimicrobium buryatense

Cloned(Commentary)

Cloned (Comment) Organism
-
 Methylotuvimicrobium buryatense

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-Xylulose 5-phosphate + phosphate Methylotuvimicrobium buryatense the phosphoketolase pathway functions in heterofermentative bacteria where carbon flux through two sugar catabolic pathways to mixed acids (lactic acid and acetic acid) increases cellular ATP production. This pathway also serves as an alternative route to produce acetyl-CoA that bypasses the CO2 lost through pyruvate decarboxylation in the Embden-Meyerhof-Parnas pathway Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
 ?
D-Xylulose 5-phosphate + phosphate Methylotuvimicrobium buryatense 5GB1S the phosphoketolase pathway functions in heterofermentative bacteria where carbon flux through two sugar catabolic pathways to mixed acids (lactic acid and acetic acid) increases cellular ATP production. This pathway also serves as an alternative route to produce acetyl-CoA that bypasses the CO2 lost through pyruvate decarboxylation in the Embden-Meyerhof-Parnas pathway Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Methylotuvimicrobium buryatense
-
-
-
Methylotuvimicrobium buryatense 5GB1S
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-
-

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:methane-grown cell
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 Methylotuvimicrobium buryatense
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culture condition:methanol-grown cell
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 Methylotuvimicrobium buryatense
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Xylulose 5-phosphate + phosphate
-
 Methylotuvimicrobium buryatense Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
 ?
D-Xylulose 5-phosphate + phosphate the phosphoketolase pathway functions in heterofermentative bacteria where carbon flux through two sugar catabolic pathways to mixed acids (lactic acid and acetic acid) increases cellular ATP production. This pathway also serves as an alternative route to produce acetyl-CoA that bypasses the CO2 lost through pyruvate decarboxylation in the Embden-Meyerhof-Parnas pathway Methylotuvimicrobium buryatense Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
 ?
D-Xylulose 5-phosphate + phosphate
-
 Methylotuvimicrobium buryatense 5GB1S Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
 ?
D-Xylulose 5-phosphate + phosphate the phosphoketolase pathway functions in heterofermentative bacteria where carbon flux through two sugar catabolic pathways to mixed acids (lactic acid and acetic acid) increases cellular ATP production. This pathway also serves as an alternative route to produce acetyl-CoA that bypasses the CO2 lost through pyruvate decarboxylation in the Embden-Meyerhof-Parnas pathway Methylotuvimicrobium buryatense 5GB1S Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
 ?

General Information

General Information Comment Organism
metabolism the phosphoketolase pathway functions in heterofermentative bacteria where carbon flux through two sugar catabolic pathways to mixed acids (lactic acid and acetic acid) increases cellular ATP production. This pathway also serves as an alternative route to produce acetyl-CoA that bypasses the CO2 lost through pyruvate decarboxylation in the Embden-Meyerhof-Parnas pathway Methylotuvimicrobium buryatense