Literature summary for 4.1.2.9 extracted from

Petrareanu, G.; Balasu, M.; Vacaru, A.; Munteanu, C.; Ionescu, A.; Matei, I.; Szedlacsek, S.
Phosphoketolases from Lactococcus lactis, Leuconostoc mesenteroides and Pseudomonas aeruginosa Dissimilar sequences, similar substrates but distinct enzymatic characteristics (2014), Appl. Microbiol. Biotechnol., 98, 7855-7867 .

Search for more literature for 4.1.2.9

Cloned(Commentary)

Cloned (Comment)	Organism
cloned in a prokaryotic vector, and the encoded protein is expressed in Escherichia coli	Leuconostoc mesenteroides subsp. mesenteroides
cloned in a prokaryotic vector, and the encoded protein is expressed in Escherichia coli	Pseudomonas aeruginosa
cloned in a prokaryotic vector, and the encoded protein is expressed in Escherichia coli	Lactococcus lactis subsp. lactis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.035	-	D-xylulose 5-phosphate	pH 6.5, 37°C	Pseudomonas aeruginosa
1.76	-	D-fructose 6-phosphate	pH 6.5, 37°C	Pseudomonas aeruginosa
2.02	-	D-xylulose 5-phosphate	pH 6.5, 37°C	Leuconostoc mesenteroides subsp. mesenteroides
3.04	-	D-xylulose 5-phosphate	pH 6.5, 37°C	Lactococcus lactis subsp. lactis
9.89	-	D-fructose 6-phosphate	pH 6.5, 37°C	Lactococcus lactis subsp. lactis
45.96	-	D-fructose 6-phosphate	pH 6.5, 37°C	Leuconostoc mesenteroides subsp. mesenteroides

Organism

Organism	UniProt	Comment	Textmining
Lactococcus lactis subsp. lactis	Q9CFH4	-	-
Lactococcus lactis subsp. lactis IL1403	Q9CFH4	-	-
Leuconostoc mesenteroides subsp. mesenteroides	-	-	-
Leuconostoc mesenteroides subsp. mesenteroides J18	-	-	-
Pseudomonas aeruginosa	Q9HY13	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Leuconostoc mesenteroides subsp. mesenteroides
-	Pseudomonas aeruginosa
-	Lactococcus lactis subsp. lactis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-Fructose 6-phosphate + phosphate	the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for D-fructose 6-phosphate	Pseudomonas aeruginosa	Acetyl phosphate + D-erythrose 4-phosphate + H2O	-	?
D-Fructose 6-phosphate + phosphate	the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for D-fructose 6-phosphate	Lactococcus lactis subsp. lactis	Acetyl phosphate + D-erythrose 4-phosphate + H2O	-	?
D-Fructose 6-phosphate + phosphate	the bifunctional enzyme shows activity with fructose-6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for fructose-6-phosphate	Leuconostoc mesenteroides subsp. mesenteroides	Acetyl phosphate + D-erythrose 4-phosphate + H2O	-	?
D-Fructose 6-phosphate + phosphate	the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for D-fructose 6-phosphate	Lactococcus lactis subsp. lactis IL1403	Acetyl phosphate + D-erythrose 4-phosphate + H2O	-	?
D-Fructose 6-phosphate + phosphate	the bifunctional enzyme shows activity with fructose-6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for fructose-6-phosphate	Leuconostoc mesenteroides subsp. mesenteroides J18	Acetyl phosphate + D-erythrose 4-phosphate + H2O	-	?
D-Xylulose 5-phosphate + phosphate	the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for D-fructose 6-phosphate	Pseudomonas aeruginosa	Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O	-	?
D-Xylulose 5-phosphate + phosphate	the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for fructose-6-phosphate	Lactococcus lactis subsp. lactis	Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O	-	?
D-Xylulose 5-phosphate + phosphate	the bifunctional enzyme shows activity with fructose-6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for fructose-6-phosphate	Leuconostoc mesenteroides subsp. mesenteroides	Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O	-	?
D-Xylulose 5-phosphate + phosphate	the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for fructose-6-phosphate	Lactococcus lactis subsp. lactis IL1403	Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O	-	?
D-Xylulose 5-phosphate + phosphate	the bifunctional enzyme shows activity with fructose-6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for fructose-6-phosphate	Leuconostoc mesenteroides subsp. mesenteroides J18	Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
13.1	-	D-fructose 6-phosphate	pH 6.5, 37°C	Pseudomonas aeruginosa
13.2	-	D-xylulose 5-phosphate	pH 6.5, 37°C	Pseudomonas aeruginosa
44.2	-	D-fructose 6-phosphate	pH 6.5, 37°C	Leuconostoc mesenteroides subsp. mesenteroides
117.8	-	D-fructose 6-phosphate	pH 6.5, 37°C	Lactococcus lactis subsp. lactis
431.8	-	D-xylulose 5-phosphate	pH 6.5, 37°C	Leuconostoc mesenteroides subsp. mesenteroides
587.8	-	D-xylulose 5-phosphate	pH 6.5, 37°C	Lactococcus lactis subsp. lactis

Cofactor

Cofactor	Comment	Organism	Structure
thiamine diphosphate	-	Leuconostoc mesenteroides subsp. mesenteroides
thiamine diphosphate	-	Pseudomonas aeruginosa
thiamine diphosphate	-	Lactococcus lactis subsp. lactis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.96	-	D-fructose 6-phosphate	pH 6.5, 37°C	Leuconostoc mesenteroides subsp. mesenteroides
7.43	-	D-fructose 6-phosphate	pH 6.5, 37°C	Pseudomonas aeruginosa
11.9	-	D-fructose 6-phosphate	pH 6.5, 37°C	Lactococcus lactis subsp. lactis
193.15	-	D-xylulose 5-phosphate	pH 6.5, 37°C	Lactococcus lactis subsp. lactis
213.43	-	D-xylulose 5-phosphate	pH 6.5, 37°C	Leuconostoc mesenteroides subsp. mesenteroides
378.62	-	D-xylulose 5-phosphate	pH 6.5, 37°C	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)