Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) RIL cells | Lactococcus lactis |
expressed in Escherichia coli BL21(DE3) RIL cells | Lactococcus lactis |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 17% (w/v) PEG 3350 and 0.2 M NaSCN, at 18°C | Lactococcus lactis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | cofactor-bound Mg2+ is required for enzymatic activity | Lactococcus lactis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
95727 | - |
x * 95727, calculated from amino acid sequence | Lactococcus lactis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus lactis | - |
- |
- |
Lactococcus lactis | - |
subsp. lactis | - |
Lactococcus lactis IL 1403 | - |
- |
- |
Lactococcus lactis IL 1403 | - |
subsp. lactis | - |
Purification (Comment) | Organism |
---|---|
HisTrap HP column chromatography and Superdex 200 gel filtration | Lactococcus lactis |
HisTrap Ni2+ metal affinity column chromatography and Superdex 200 gel filtration | Lactococcus lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Fructose 6-phosphate + phosphate | the enzyme has higher activity with D-xylulose 5-phosphate than D-fructose 6-phosphate | Lactococcus lactis | Acetyl phosphate + D-erythrose 4-phosphate + H2O | - |
? | |
D-Fructose 6-phosphate + phosphate | the specificity constant for D-xylulose 5-phosphate is significantly higher than that for D-fructose 6-phosphate | Lactococcus lactis | Acetyl phosphate + D-erythrose 4-phosphate + H2O | - |
? | |
D-Fructose 6-phosphate + phosphate | the enzyme has higher activity with D-xylulose 5-phosphate than D-fructose 6-phosphate | Lactococcus lactis IL 1403 | Acetyl phosphate + D-erythrose 4-phosphate + H2O | - |
? | |
D-Fructose 6-phosphate + phosphate | the specificity constant for D-xylulose 5-phosphate is significantly higher than that for D-fructose 6-phosphate | Lactococcus lactis IL 1403 | Acetyl phosphate + D-erythrose 4-phosphate + H2O | - |
? | |
D-Xylulose 5-phosphate + phosphate | the specificity constant for D-xylulose 5-phosphate is significantly higher than that for D-fructose 6-phosphate | Lactococcus lactis | Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O | - |
? | |
D-Xylulose 5-phosphate + phosphate | the specificity constant for D-xylulose 5-phosphate is significantly higher than that for D-fructose 6-phosphate | Lactococcus lactis IL 1403 | Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O | - |
? | |
D-xylulose 5-phosphate + phosphate | most specific substrate | Lactococcus lactis | acetyl phosphate + D-glyceraldehyde 3-phosphate | - |
? | |
D-xylulose 5-phosphate + phosphate | most specific substrate | Lactococcus lactis IL 1403 | acetyl phosphate + D-glyceraldehyde 3-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 95727, calculated from amino acid sequence | Lactococcus lactis |
Synonyms | Comment | Organism |
---|---|---|
D-xylulose 5-phosphate phosphoketolase | - |
Lactococcus lactis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Lactococcus lactis | |
thiamine diphosphate | dependent on | Lactococcus lactis |