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Literature summary for 4.1.2.63 extracted from

  • Foulon, V.; Sniekers, M.; Huysmans, E.; Asselberghs, S.; Mahieu, V.; Mannaerts, G.P.; Van Veldhoven, P.P.; Casteels, M.
    Breakdown of 2-hydroxylated straight chain fatty acids via peroxisomal 2-hydroxyphytanoyl-CoA lyase a revised pathway for the alpha-oxidation of straight chain fatty acids (2005), J. Biol. Chem., 280, 9802-9812 .
    View publication on PubMed
Search for more literature for 4.1.2.63

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae strain CB80 Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited in the presence of 2-keto-octanoate, 2-hydroxyhexadecanoic acid, 2-methylhexadecanoyl-CoA, 2-methylhexadecanoic acid, 3-methylhexadecanoyl-CoA, 3-hydroxy-3-methylhexadecanoyl-CoA, or 3-hydroxy-2-methylhexadecanoyl-CoA. 2-Hydroxy straight chain acyl-CoA esters compete with 2-hydroxy-3-methyl-branched acyl-CoAs for cleavage by the enzyme Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome
-
 Rattus norvegicus 5777
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-hydroxyphytanoyl-CoA Rattus norvegicus
-
 pristanal + formyl-CoA
-
 ?
a 2-hydroxy-3-methyl-Cn-fatty-acyl-CoA Rattus norvegicus
-
 a 2-methyl-branched Cn-1-fatty aldehyde + formyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus Q8CHM7
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
hepatocyte
-
 Rattus norvegicus
-
liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxy-3-methylhexadecanoyl-CoA
-
 Rattus norvegicus 2-hydroxy-3-methylpentadecanal + formyl-CoA
-
 ?
2-hydroxyoctadecanoyl-CoA
-
 Rattus norvegicus heptadecanal + formyl-CoA
-
 ?
2-hydroxyphytanoyl-CoA
-
 Rattus norvegicus pristanal + formyl-CoA
-
 ?
a 2-hydroxy-3-methyl-Cn-fatty-acyl-CoA
-
 Rattus norvegicus a 2-methyl-branched Cn-1-fatty aldehyde + formyl-CoA
-
 ?
additional information no activity toward 3-methylhexadecanoyl-CoA, hexadecanoyl-CoA, 3-hydroxyhexadecanoyl-CoA, octadecanoyl-CoA, 2-hydroxy-3-methylhexadecanoic acid, 3-methylhexadecanoic acid, hexadecanoic acid, and 2-hydroxyhexadecanoic acid. The addition of 0.05 mM 2-hydroxyhexadecanoyl-CoA or 2-hydroxyoctadecanoyl-CoA to incubations with 0.01 mM 2-hydroxy-3-methylhexadecanoyl-CoA reduces the cleavage rates to a major extent Rattus norvegicus ?
-
-

Subunits

Subunits Comment Organism
? x * 63000, SDS-PAGE Rattus norvegicus

Synonyms

Synonyms Comment Organism
2-HPCL
-
 Rattus norvegicus
2-hydroxyphytanoyl-CoA lyase
-
 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
ATP dependent on. No oxidation is detected upon the addition of GTP or AMP Rattus norvegicus
CoA dependent on. CoA cannot be replaced by desulfo-CoA or dephospho-CoA Rattus norvegicus
NAD+ dependent on. Extra addition of NAD+ results in a 20% increase of total oxidation Rattus norvegicus
thiamine diphosphate dependent on Rattus norvegicus

General Information

General Information Comment Organism
metabolism essential enzyme in the peroxisomal alpha-oxidation of 3-methyl-branched fatty acids such as phytanic acid Rattus norvegicus