Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta(DE3) pLysS cells | Escherichia coli |
expressed in Escherichia coli Rosetta(DE3) pLysS cells | Rhodospirillum rubrum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.229 | - |
5-deoxy-D-ribulose 1-phosphate | at pH 7.8, temperature not specified in the publication | Rhodospirillum rubrum | |
0.275 | - |
S-methyl-5-thio-D-ribulose 1-phosphate | at pH 7.8, temperature not specified in the publication | Escherichia coli | |
0.288 | - |
S-methyl-5-thio-D-ribulose 1-phosphate | at pH 7.8, temperature not specified in the publication | Rhodospirillum rubrum | |
0.598 | - |
5-deoxy-D-ribulose 1-phosphate | at pH 7.8, temperature not specified in the publication | Escherichia coli | |
0.827 | - |
ribulose 1-phosphate | at pH 7.8, temperature not specified in the publication | Escherichia coli | |
7 | - |
ribulose 1-phosphate | Km above 7.0 mM, at pH 7.8, temperature not specified in the publication | Rhodospirillum rubrum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-deoxy-D-ribulose 1-phosphate | Escherichia coli | - |
glycerone phosphate + acetaldehyde | - |
? | |
5-deoxy-D-ribulose 1-phosphate | Rhodospirillum rubrum | - |
glycerone phosphate + acetaldehyde | - |
? | |
5-deoxy-D-ribulose 1-phosphate | Escherichia coli ATCC 25922 | - |
glycerone phosphate + acetaldehyde | - |
? | |
S-methyl-5-thio-D-ribulose 1-phosphate | Escherichia coli | - |
glycerone phosphate + (2-methylthio)acetaldehyde | - |
? | |
S-methyl-5-thio-D-ribulose 1-phosphate | Rhodospirillum rubrum | - |
glycerone phosphate + (2-methylthio)acetaldehyde | - |
? | |
S-methyl-5-thio-D-ribulose 1-phosphate | Escherichia coli ATCC 25922 | - |
glycerone phosphate + (2-methylthio)acetaldehyde | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli ATCC 25922 | - |
- |
- |
Rhodospirillum rubrum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nickel affinity chromatography | Escherichia coli |
nickel affinity chromatography | Rhodospirillum rubrum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-deoxy-D-ribulose 1-phosphate | - |
Escherichia coli | glycerone phosphate + acetaldehyde | - |
? | |
5-deoxy-D-ribulose 1-phosphate | - |
Rhodospirillum rubrum | glycerone phosphate + acetaldehyde | - |
? | |
5-deoxy-D-ribulose 1-phosphate | - |
Escherichia coli ATCC 25922 | glycerone phosphate + acetaldehyde | - |
? | |
ribulose 1-phosphate | - |
Escherichia coli | ? | - |
? | |
ribulose 1-phosphate | - |
Rhodospirillum rubrum | ? | - |
? | |
ribulose 1-phosphate | - |
Escherichia coli ATCC 25922 | ? | - |
? | |
S-methyl-5-thio-D-ribulose 1-phosphate | - |
Escherichia coli | glycerone phosphate + (2-methylthio)acetaldehyde | - |
? | |
S-methyl-5-thio-D-ribulose 1-phosphate | - |
Rhodospirillum rubrum | glycerone phosphate + (2-methylthio)acetaldehyde | - |
? | |
S-methyl-5-thio-D-ribulose 1-phosphate | - |
Escherichia coli ATCC 25922 | glycerone phosphate + (2-methylthio)acetaldehyde | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ald2 | - |
Escherichia coli |
ald2 | - |
Rhodospirillum rubrum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.44 | - |
ribulose 1-phosphate | at pH 7.8, temperature not specified in the publication | Rhodospirillum rubrum | |
0.94 | - |
S-methyl-5-thio-D-ribulose 1-phosphate | at pH 7.8, temperature not specified in the publication | Rhodospirillum rubrum | |
1.94 | - |
5-deoxy-D-ribulose 1-phosphate | at pH 7.8, temperature not specified in the publication | Rhodospirillum rubrum | |
8.7 | - |
ribulose 1-phosphate | at pH 7.8, temperature not specified in the publication | Escherichia coli | |
22 | - |
S-methyl-5-thio-D-ribulose 1-phosphate | at pH 7.8, temperature not specified in the publication | Escherichia coli | |
22 | - |
5-deoxy-D-ribulose 1-phosphate | at pH 7.8, temperature not specified in the publication | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
ribulose 1-phosphate | kcat_Km value below 0.06 1/sec*mM, at pH 7.8, temperature not specified in the publication | Rhodospirillum rubrum | |
1.7 | - |
S-methyl-5-thio-D-ribulose 1-phosphate | at pH 7.8, temperature not specified in the publication | Rhodospirillum rubrum | |
8.5 | - |
5-deoxy-D-ribulose 1-phosphate | at pH 7.8, temperature not specified in the publication | Rhodospirillum rubrum | |
11 | - |
ribulose 1-phosphate | at pH 7.8, temperature not specified in the publication | Escherichia coli | |
37 | - |
5-deoxy-D-ribulose 1-phosphate | at pH 7.8, temperature not specified in the publication | Escherichia coli | |
78 | - |
S-methyl-5-thio-D-ribulose 1-phosphate | at pH 7.8, temperature not specified in the publication | Escherichia coli |