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Literature summary for 4.1.2.55 extracted from

  • Archer, R.M.; Royer, S.F.; Mahy, W.; Winn, C.L.; Danson, M.J.; Bull, S.D.
    Syntheses of 2-keto-3-deoxy-D-xylonate and 2-keto-3-deoxy-L-arabinonate as stereochemical probes for demonstrating the metabolic promiscuity of Sulfolobus solfataricus towards D-xylose and L-arabinose (2013), Chemistry, 19, 2895-2902.
    View publication on PubMed
Search for more literature for 4.1.2.55

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
9.9
-
 2-dehydro-3-deoxy-D-galactonate pH 6.5, 70°C Saccharolobus solfataricus
17.6
-
 2-dehydro-3-deoxy-L-arabinonate pH 6.5, 50°C Saccharolobus solfataricus
25.7
-
 2-dehydro-3-deoxy-D-gluconate pH 6.5, 70°C Saccharolobus solfataricus
33.3
-
 2-dehydro-3-deoxy-D-xylonate pH 6.5, 50°C Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-dehydro-3-deoxy-D-galactonate
-
 Saccharolobus solfataricus pyruvate + D-glyceraldehyde
-
 ?
2-dehydro-3-deoxy-D-gluconate
-
 Saccharolobus solfataricus pyruvate + D-glyceraldehyde
-
 ?
2-dehydro-3-deoxy-D-xylonate
-
 Saccharolobus solfataricus pyruvate + glycolaldehyde
-
 ?
2-dehydro-3-deoxy-L-arabinonate
-
 Saccharolobus solfataricus pyruvate + glycolaldehyde
-
 ?

Synonyms

Synonyms Comment Organism
KDG-aldolase
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 assay at, 2-dehydro-3-deoxy-D-xylonate and 2-dehydro-3-deoxy-L-arabinonate are less thermally stable than these C6 analogues (2-dehydro-3-deoxy-D-gluconate and 2-dehydro-3-deoxy-D-galactonate), and so their kinetic assays are carried out at 50°C Saccharolobus solfataricus
70
-
 assay with 2-dehydro-3-deoxy-D-gluconate or 2-dehydro-3-deoxy-D-galactonate as substrates Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.8
-
 2-dehydro-3-deoxy-D-galactonate pH 6.5, 70°C Saccharolobus solfataricus
9.3
-
 2-dehydro-3-deoxy-L-arabinonate pH 6.5, 50°C Saccharolobus solfataricus
15
-
 2-dehydro-3-deoxy-D-xylonate pH 6.5, 50°C Saccharolobus solfataricus
28.2
-
 2-dehydro-3-deoxy-D-gluconate pH 6.5, 70°C Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.45
-
 2-dehydro-3-deoxy-D-xylonate pH 6.5, 50°C Saccharolobus solfataricus
0.53
-
 2-dehydro-3-deoxy-L-arabinonate pH 6.5, 50°C Saccharolobus solfataricus
0.7
-
 2-dehydro-3-deoxy-D-galactonate pH 6.5, 70°C Saccharolobus solfataricus
1.1
-
 2-dehydro-3-deoxy-D-gluconate pH 6.5, 70°C Saccharolobus solfataricus