Literature summary for 4.1.2.55 extracted from

Theodossis, A.; Walden, H.; Westwick, E.J.; Connaris, H.; Lamble, H.J.; Hough, D.W.; Danson, M.J.; Taylor, G.L.
The structural basis for substrate promiscuity in 2-keto-3-deoxygluconate aldolase from the Entner-Doudoroff pathway in Sulfolobus solfataricus (2004), J. Biol. Chem., 279, 43886-43892.

Crystallization (Commentary)

Crystallization (Comment)	Organism
trapping of Schiff base complexes with natural substrates pyruvate, 2-dehydro-3-deoxy-D-gluconate, 2-dehydro-3-deoxy-D-galactonate and pyruvate plus D-glyceraldehyde at physiological pH by rapid soaking at low temperature followed by flash freezing prior to X-ray data collection. The complexes explain the substrate promiscuity of the enzyme with a rigid ligand-binding site able to accommodate both 2-dehydro-3-deoxy-D-gluconate and 2-dehydro-3-deoxy-D-galactonate	Saccharolobus solfataricus

Crystallization (Comment)

Organism

trapping of Schiff base complexes with natural substrates pyruvate, 2-dehydro-3-deoxy-D-gluconate, 2-dehydro-3-deoxy-D-galactonate and pyruvate plus D-glyceraldehyde at physiological pH by rapid soaking at low temperature followed by flash freezing prior to X-ray data collection. The complexes explain the substrate promiscuity of the enzyme with a rigid ligand-binding site able to accommodate both 2-dehydro-3-deoxy-D-gluconate and 2-dehydro-3-deoxy-D-galactonate

Saccharolobus solfataricus

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	O54288	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-dehydro-3-deoxy-D-galactonate	lack of stereoselectivity in the condensation reaction of pyruvate and D-glyceraldehyde, forming a mixture of 2-dehydro-3-deoxy-D-gluconate and 2-dehydro-3-deoxy-D-galactonate	Saccharolobus solfataricus	pyruvate + D-glyceraldehyde	-	r
2-dehydro-3-deoxy-D-gluconate	lack of stereoselectivity in the condensation reaction of pyruvate and D-glyceraldehyde, forming a mixture of 2-dehydro-3-deoxy-D-gluconate and 2-dehydro-3-deoxy-D-galactonate	Saccharolobus solfataricus	pyruvate + D-glyceraldehyde	-	r

Synonyms

Synonyms	Comment	Organism
KDGA	-	Saccharolobus solfataricus

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Release 2023.1 (January 2023)