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Literature summary for 4.1.2.55 extracted from

  • Lamble, H.J.; Heyer, N.I.; Bull, S.D.; Hough, D.W.; Danson, M.J.
    Metabolic pathway promiscuity in the archaeon Sulfolobus solfataricus revealed by studies on glucose dehydrogenase and 2-keto-3-deoxygluconate aldolase (2003), J. Biol. Chem., 278, 34066-34072.
    View publication on PubMed
Search for more literature for 4.1.2.55

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
9.9
-
 2-dehydro-3-deoxy-D-galactonate pH 6.0, 60°C Saccharolobus solfataricus
25.7
-
 2-dehydro-3-deoxy-D-gluconate pH 6.0, 60°C Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-dehydro-3-deoxy-D-galactonate Saccharolobus solfataricus the same enzyme is responsible for the catabolism of both glucose and galactose pyruvate + D-glyceraldehyde
-
 ?
2-dehydro-3-deoxy-D-gluconate Saccharolobus solfataricus the same enzyme is responsible for the catabolism of both glucose and galactose pyruvate + D-glyceraldehyde
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus O54288
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-dehydro-3-deoxy-D-galactonate the same enzyme is responsible for the catabolism of both glucose and galactose Saccharolobus solfataricus pyruvate + D-glyceraldehyde
-
 ?
2-dehydro-3-deoxy-D-gluconate the same enzyme is responsible for the catabolism of both glucose and galactose Saccharolobus solfataricus pyruvate + D-glyceraldehyde
-
 ?
pyruvate + D-glyceraldehyde the enzyme shows a lack of facial selectivity, yielding approximately equal quantities of 2-keto-3-deoxy-D-gluconate and 2-keto-3-deoxy-D-galactonate Saccharolobus solfataricus 2-dehydro-3-deoxy-D-galactonate
-
 r
pyruvate + D-glyceraldehyde the enzyme shows a lack of facial selectivity, yielding approximately equal quantities of 2-keto-3-deoxy-D-gluconate and 2-keto-3-deoxy-D-galactonate Saccharolobus solfataricus 2-dehydro-3-deoxy-D-gluconate
-
 r

Synonyms

Synonyms Comment Organism
2-keto-3-deoxygluconate aldolase
-
 Saccharolobus solfataricus
KDG aldolase
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 assay at Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.8
-
 2-dehydro-3-deoxy-D-galactonate pH 6.0, 60°C Saccharolobus solfataricus
28.2
-
 2-dehydro-3-deoxy-D-gluconate pH 6.0, 60°C Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.7
-
 2-dehydro-3-deoxy-D-galactonate pH 6.0, 60°C Saccharolobus solfataricus
1.1
-
 2-dehydro-3-deoxy-D-gluconate pH 6.0, 60°C Saccharolobus solfataricus