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Literature summary for 4.1.2.54 extracted from

  • Hilditch, S.; Berghäll, S.; Kalkkinen, N.; Penttilä, M.; Richard, P.
    The missing link in the fungal D-galacturonate pathway: identification of the L-threo-3-deoxy-hexulosonate aldolase (2007), J. Biol. Chem., 282, 26195-26201.
    View publication on PubMed
Search for more literature for 4.1.2.54

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae as a histidine-tagged protein Trichoderma reesei

Inhibitors

Inhibitors Comment Organism Structure
D-glyceraldehyde inhibits reverse reaction with pyruvate and L-glyceraldehyde Trichoderma reesei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 pyruvate pH 7.0, 30°C Trichoderma reesei
1.2
-
 L-glyceraldehyde pH 7.0, 30°C Trichoderma reesei
3.5
-
 2-dehydro-3-deoxy-L-galactonate pH 7.0, 30°C Trichoderma reesei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33407
-
 x * 33407, calculated from sequence Trichoderma reesei
37000
-
 x * 37000, SDS-PAGE Trichoderma reesei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-dehydro-3-deoxy-L-galactonate Trichoderma reesei the enzyme is part of the fungal pathway of D-galacturonate degradation pyruvate + L-glyceraldehyde
-
 r

Organism

Organism UniProt Comment Textmining
Trichoderma reesei A6Y9S5 i.e. Trichoderma reesei
-

Purification (Commentary)

Purification (Comment) Organism
-
 Trichoderma reesei

Source Tissue

Source Tissue Comment Organism Textmining
mycelium the L-threo-3-deoxy-hexulosonate aldolase activity is induced during growth on D-galacturonate Trichoderma reesei
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-dehydro-3-deoxy-L-galactonate the enzyme is part of the fungal pathway of D-galacturonate degradation Trichoderma reesei pyruvate + L-glyceraldehyde
-
 r
2-dehydro-3-deoxy-L-galactonate the reverse reaction is significantly stronger with L-glyceraldehyde as a substrate than with D-glyceraldehyde. No activity with D-threo-3-deoxy-hexulosonate Trichoderma reesei pyruvate + L-glyceraldehyde
-
 r
additional information the reaction with D-glyceraldehyde, dihydroxyacetone, diacetyl, acetone, glyoxal, acetaldehyde or formaldehyde plus pyruvate is below the detection limit Trichoderma reesei ?
-
 ?
pyruvate + glycolaldehyde
-
 Trichoderma reesei ?
-
 ?
pyruvate + L-glyceraldehyde
-
 Trichoderma reesei 2-dehydro-3-deoxy-L-galactonate
-
 r
pyruvate + methylglyoxal
-
 Trichoderma reesei ?
-
 ?

Subunits

Subunits Comment Organism
? x * 37000, SDS-PAGE Trichoderma reesei
? x * 33407, calculated from sequence Trichoderma reesei

Synonyms

Synonyms Comment Organism
LGA1
-
 Trichoderma reesei

Expression

Organism Comment Expression
Trichoderma reesei induced in Hypocrea jecorina during growth on D-galacturonate up

General Information

General Information Comment Organism
malfunction the deletion of the gene results in a strain unable to grow on D-galacturonate and accumulating L-threo-3-deoxy-hexulosonate Trichoderma reesei
physiological function the enzyme is induced during growth on D-galacturonate Trichoderma reesei