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Literature summary for 4.1.2.53 extracted from

  • Hernandez, K.; Gomez, A.; Joglar, J.; Bujons, J.; Parella, T.; Clapes, P.
    2-Keto-3-deoxy-L-rhamnonate aldolase (YfaU) as catalyst in aldol additions of pyruvate to amino aldehyde derivatives (2017), Adv. Synth. Catal., 359, 2090-2100 .
No PubMed abstract available
Search for more literature for 4.1.2.53

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli M-15[pREP-4] cells Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ natural metal cofactor Escherichia coli
Ni2+ activates better than Mg2+ Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyruvate + (S)-lactaldehyde Escherichia coli
-
 2-dehydro-3-deoxy-L-rhamnonate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P76469
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap column chromatography, and Superdex 200 gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyl (1-oxobutan-2-yl)carbamate + 2-oxopropanoate
-
 Escherichia coli (3S)-4-[[(benzyloxy)carbonyl]amino]-3-hydroxyhexanoate + (3R)-4-[[(benzyloxy)carbonyl]amino]-3-hydroxyhexanoate
-
 ?
benzyl (1-oxopropan-2-yl)carbamate + 2-oxopropanoate
-
 Escherichia coli (3S)-4-[[(benzyloxy)carbonyl]amino]-3-hydroxypentanoate + (3R)-4-[[(benzyloxy)carbonyl]amino]-3-hydroxypentanoate
-
 ?
benzyl (2-oxoethyl)carbamate + 2-oxopropanoate
-
 Escherichia coli (3S)-4-[[(benzyloxy)carbonyl]amino]-3-hydroxybutanoate + (3R)-4-[[(benzyloxy)carbonyl]amino]-3-hydroxybutanoate
-
 ?
benzyl (3-methyl-1-oxobutan-2-yl)carbamate + 2-oxopropanoate
-
 Escherichia coli (3S)-4-[[(benzyloxy)carbonyl]amino]-3-hydroxy-5-methylhexanoate + (3R)-4-[[(benzyloxy)carbonyl]amino]-3-hydroxy-5-methylhexanoate
-
 ?
benzyl (3-oxopropyl)carbamate + 2-oxopropanoate
-
 Escherichia coli (3R)-5-[[(benzyloxy)carbonyl]amino]-3-hydroxypentanoate + (3S)-5-[[(benzyloxy)carbonyl]amino]-3-hydroxypentanoate
-
 ?
benzyl (4-methyl-1-oxopentan-2-yl)carbamate + 2-oxopropanoate
-
 Escherichia coli (3S)-4-[[(benzyloxy)carbonyl]amino]-3-hydroxy-6-methylheptanoate + (3R)-4-[[(benzyloxy)carbonyl]amino]-3-hydroxy-6-methylheptanoate
-
 ?
benzyl 2-formylpyrrolidine-1-carboxylate + 2-oxopropanoate
-
 Escherichia coli (4S)-4-[1-[(benzyloxy)carbonyl]pyrrolidin-2-yl]-4-hydroxy-2-oxobutanoate + (4R)-4-[1-[(benzyloxy)carbonyl]pyrrolidin-2-yl]-4-hydroxy-2-oxobutanoate
-
 ?
benzyl [(3S)-3-methyl-1-oxopentan-2-yl]carbamate + 2-oxopropanoate
-
 Escherichia coli (3S,5R)-4-[[(benzyloxy)carbonyl]amino]-3-hydroxy-5-methylheptanoate + (3R,5R)-4-[[(benzyloxy)carbonyl]amino]-3-hydroxy-5-methylheptanoate
-
 ?
pyruvate + (S)-lactaldehyde
-
 Escherichia coli 2-dehydro-3-deoxy-L-rhamnonate
-
 ?

Synonyms

Synonyms Comment Organism
YfaU
-
 Escherichia coli