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Literature summary for 4.1.2.52 extracted from
- Structure and mechanism of HpcH: a metal ion dependent class II aldolase from the homoprotocatechuate degradation pathway of Escherichia coli (2007), J. Mol. Biol., 373, 866-876.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| HpcH gene expressed in Escherichia coli strain B834 (DE3) | Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystallization of apo-HpcH, because divalent metal ion is lost during the purification process, resulting in preparation of the inactive apo form of the enzyme, crystals grown using the hanging-drop vapour diffusion method | Escherichia coli |
| HpcHMg2+oxamate crystals grown with the addition of 10 mM magnesium chloride and sodium oxamate (substrate analogue), elucidation of active site architecture | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
- |
Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-hydroxy-2-oxo-heptanedioate | Escherichia coli | - |
pyruvate + succinic semialdehyde | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| metal affinity and size exclusion chromatography, Superdex 200 column used | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-hydroxy-2-oxo-heptanedioate | - |
Escherichia coli | pyruvate + succinic semialdehyde | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 28000 | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase | - |
Escherichia coli |
| HpcH | - |
Escherichia coli |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | enzyme is part of homoprotocatechuate degradation pathway | Escherichia coli |
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Release 2023.1 (January 2023)
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