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Literature summary for 4.1.2.51 extracted from

  • Reher, M.; Fuhrer, T.; Bott, M.; Schoenheit, P.
    The nonphosphorylative Entner-Doudoroff pathway in the thermoacidophilic euryarchaeon Picrophilus torridus involves a novel 2-keto-3-deoxygluconate-specific aldolase (2010), J. Bacteriol., 192, 964-974.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.2.51

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Picrophilus torridus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 2-dehydro-3-deoxy-D-gluconate pH 6.2, 60°C, enzyme purified from Picrophilus torridus Picrophilus torridus
0.4
-
 2-dehydro-3-deoxy-D-gluconate pH 6.2, 60°C, recombinant enzyme Picrophilus torridus
2.5
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate pH 6.2, 60°C, recombinant enzyme Picrophilus torridus
2.7
-
 pyruvate pH 6.2, 60°C Picrophilus torridus
4.6
-
 D-glyceraldehyde pH 6.2, 60°C Picrophilus torridus
8
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate pH 6.2, 60°C, enzyme purified from Picrophilus torridus Picrophilus torridus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 4 * 32000, SDS-PAGE Picrophilus torridus
120000
-
 gel filtration Picrophilus torridus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-dehydro-3-deoxy-D-gluconate Picrophilus torridus the enzyme from the archaeon Picrophilus torridus is involved in D-glucose and D-galactose catabolism via the nonphosphorylative variant of the Entner-Doudoroff pathway pyruvate + D-glyceraldehyde
-
 ?

Organism

Organism UniProt Comment Textmining
Picrophilus torridus Q6KZI8
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Picrophilus torridus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
48
-
 pH 6.2, 60°C, substrate: 2-dehydro-3-deoxy-D-gluconate Picrophilus torridus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-dehydro-3-deoxy-D-galactonate
-
 Picrophilus torridus pyruvate + D-glyceraldehyde
-
 ?
2-dehydro-3-deoxy-D-galactonate the enzyme shows substrate promiscuity, as measured in the direction of aldol synthesis. The enzyme catalyzes the formation of both 2-dehydro-3-deoxy-D-gluconate and its C-4 epimer 2-keto-3-deoxygalactonate from D- or L-glyceraldehyde and pyruvate at a similar ratio, indicating lack of stereospecific control in formation and cleavage of 2-dehydro-3-deoxy-D-gluconate and 2-dehydro-3-deoxy-D-galactonate Picrophilus torridus pyruvate + D-glyceraldehyde
-
 r
2-dehydro-3-deoxy-D-gluconate the enzyme from the archaeon Picrophilus torridus is involved in D-glucose and D-galactose catabolism via the nonphosphorylative variant of the Entner-Doudoroff pathway Picrophilus torridus pyruvate + D-glyceraldehyde
-
 ?
2-dehydro-3-deoxy-D-gluconate highly specific for 2-dehydro-3-deoxy-D-gluconate with up to 2000fold-higher catalytic efficiency compared to 2-dehydro-3-deoxy-6-phosphogluconate Picrophilus torridus pyruvate + D-glyceraldehyde
-
 ?
2-dehydro-3-deoxy-D-gluconate 6-phosphate highly specific for 2-dehydro-3-deoxy-D-gluconate with up to 2000fold-higher catalytic efficiency compared to 2-dehydro-3-deoxy-6-phosphogluconate Picrophilus torridus pyruvate + D-glyceraldehyde 3-phosphate
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 32000, SDS-PAGE Picrophilus torridus

Synonyms

Synonyms Comment Organism
KDG-specific aldolase
-
 Picrophilus torridus
KDGA
-
 Picrophilus torridus
Pto1279
-
 Picrophilus torridus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 assay at Picrophilus torridus
65
-
-
 Picrophilus torridus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 2 h, stable Picrophilus torridus
80
-
 half-life: 20 min Picrophilus torridus
90
-
 half-life: 15 min Picrophilus torridus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.34
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate pH 6.2, 60°C, enzyme purified from Picrophilus torridus Picrophilus torridus
0.36
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate pH 6.2, 60°C, recombinant enzyme Picrophilus torridus
24.2
-
 2-dehydro-3-deoxy-D-gluconate pH 6.2, 60°C, recombinant enzyme Picrophilus torridus
26.7
-
 2-dehydro-3-deoxy-D-gluconate pH 6.2, 60°C, enzyme purified from Picrophilus torridus Picrophilus torridus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Picrophilus torridus
6.2
-
 assay at Picrophilus torridus

pH Range

pH Minimum pH Maximum Comment Organism
4.5 7.5 pH 4.5: 50% of maximal activity, pH 7.5: about 50% of maximal activity Picrophilus torridus

General Information

General Information Comment Organism
physiological function the enzyme is involved in the nonphosphorylative variant of the Entner–Doudoroff pathway Picrophilus torridus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.04
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate pH 6.2, 60°C, enzyme purified from Picrophilus torridus Picrophilus torridus
0.14
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate pH 6.2, 60°C, recombinant enzyme Picrophilus torridus
52
-
 2-dehydro-3-deoxy-D-gluconate pH 6.2, 60°C, recombinant enzyme Picrophilus torridus
89
-
 2-dehydro-3-deoxy-D-gluconate pH 6.2, 60°C, enzyme purified from Picrophilus torridus Picrophilus torridus