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Literature summary for 4.1.2.50 extracted from
- Crystallization and preliminary X-ray characterization of queD from Bacillus subtilis, an enzyme involved in queuosine biosynthesis (2008), Acta Crystallogr. Sect. F, 64, 119-122.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene queD, overexpression in Escherichia coli strain BL21 (DE3) | Bacillus subtilis |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| purified recomb inant QueD, hanging-drop vapor-diffusion method, mixing of 800 nl of protein solution containing 10 mg/ml protein and of 800 nl crystallization solution containing 25% PEG 600, 100 mM NaCl and sodium citrate buffer, pH 5.5, at 18°C, X-ray diffraction structure determination and analysis at 3.6 A resolution | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
gene queD | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant QueD from Escherichia coli strain BL21 (DE3) by anion exchange and hydrophobic inetraction chromatography | Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| QueD | formerly YkvK | Bacillus subtilis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | QueD is one of several enzymes involved in the biosynthesis of the hypermodified nucleoside queuosine. Formation of queuosine is a multistep reaction that can be divided into three stages, overview | Bacillus subtilis |
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