BRENDA - Enzyme Database show
show all sequences of 4.1.2.5

Expanding the threonine aldolase toolbox for the asymmetric synthesis of tertiary alpha-amino acids

Fesko, K.; Strohmeier, G.A.; Breinbauer, R.; Appl. Microbiol. Biotechnol. 99, 9651-9661 (2015)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
overexpression in Escherichia coli
Aeromonas veronii
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2
-
D-beta-phenylserine
25°C, pH 8.0
Aeromonas veronii
2.76
-
alpha-methyl-beta-phenylserine
25°C, pH 8.0
Aeromonas veronii
10.9
-
D-threonine
pH 8.0, temperature not specified in the publication
Aeromonas veronii
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aeromonas veronii
-
-
-
Purification (Commentary)
Commentary
Organism
-
Aeromonas veronii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-methyl-beta-phenylserine
-
746900
Aeromonas veronii
?
-
-
-
?
D-alanine + 3-nitrobenzaldehyde
23% conversion
746900
Aeromonas veronii
beta-(3-nitrophenyl)-alpha-methyl-serine
-
-
-
?
D-alanine + benzaldehyde
10% conversion
746900
Aeromonas veronii
2-amino-3-hydroxy-2-methyl-3-phenylpropanoic acid
-
-
-
?
D-alanine + hexanal
4% conversion
746900
Aeromonas veronii
2-amino-3-hydroxy-2-methyloctanoic acid
-
-
-
?
D-beta-phenylserine
-
746900
Aeromonas veronii
glycine + benzaldehyde
-
-
-
?
D-serine + 3-nitrobenzaldehyde
2% conversion
746900
Aeromonas veronii
beta-(3-nitrophenyl)-alpha-hydroxymethyl-serine
-
-
-
?
D-serine + benzaldehyde
4% conversion
746900
Aeromonas veronii
2-amino-3-hydroxy-2-hydroxymethyl-3-phenylpropanoic acid
-
-
-
?
D-threonine
-
746900
Aeromonas veronii
glycine + acetaldehyde
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.2
-
D-threonine
pH 8.0, temperature not specified in the publication
Aeromonas veronii
0.54
-
alpha-methyl-beta-phenylserine
25°C, pH 8.0
Aeromonas veronii
18.3
-
D-beta-phenylserine
25°C, pH 8.0
Aeromonas veronii
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
pyridoxal 5'-phosphate-dependent enzyme
Aeromonas veronii
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpression in Escherichia coli
Aeromonas veronii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
pyridoxal 5'-phosphate-dependent enzyme
Aeromonas veronii
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2
-
D-beta-phenylserine
25°C, pH 8.0
Aeromonas veronii
2.76
-
alpha-methyl-beta-phenylserine
25°C, pH 8.0
Aeromonas veronii
10.9
-
D-threonine
pH 8.0, temperature not specified in the publication
Aeromonas veronii
Purification (Commentary) (protein specific)
Commentary
Organism
-
Aeromonas veronii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-methyl-beta-phenylserine
-
746900
Aeromonas veronii
?
-
-
-
?
D-alanine + 3-nitrobenzaldehyde
23% conversion
746900
Aeromonas veronii
beta-(3-nitrophenyl)-alpha-methyl-serine
-
-
-
?
D-alanine + benzaldehyde
10% conversion
746900
Aeromonas veronii
2-amino-3-hydroxy-2-methyl-3-phenylpropanoic acid
-
-
-
?
D-alanine + hexanal
4% conversion
746900
Aeromonas veronii
2-amino-3-hydroxy-2-methyloctanoic acid
-
-
-
?
D-beta-phenylserine
-
746900
Aeromonas veronii
glycine + benzaldehyde
-
-
-
?
D-serine + 3-nitrobenzaldehyde
2% conversion
746900
Aeromonas veronii
beta-(3-nitrophenyl)-alpha-hydroxymethyl-serine
-
-
-
?
D-serine + benzaldehyde
4% conversion
746900
Aeromonas veronii
2-amino-3-hydroxy-2-hydroxymethyl-3-phenylpropanoic acid
-
-
-
?
D-threonine
-
746900
Aeromonas veronii
glycine + acetaldehyde
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.2
-
D-threonine
pH 8.0, temperature not specified in the publication
Aeromonas veronii
0.54
-
alpha-methyl-beta-phenylserine
25°C, pH 8.0
Aeromonas veronii
18.3
-
D-beta-phenylserine
25°C, pH 8.0
Aeromonas veronii
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.0027
-
D-threonine
pH 8.0, temperature not specified in the publication
Aeromonas veronii
0.195
-
alpha-methyl-beta-phenylserine
25°C, pH 8.0
Aeromonas veronii
9.2
-
D-beta-phenylserine
25°C, pH 8.0
Aeromonas veronii
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.0027
-
D-threonine
pH 8.0, temperature not specified in the publication
Aeromonas veronii
0.195
-
alpha-methyl-beta-phenylserine
25°C, pH 8.0
Aeromonas veronii
9.2
-
D-beta-phenylserine
25°C, pH 8.0
Aeromonas veronii
Other publictions for EC 4.1.2.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746894
Bulut
Development of a growth-depen ...
Pseudomonas putida
Appl. Microbiol. Biotechnol.
99
5875-5883
2015
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1
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1
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1
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1
-
1
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-
-
-
-
-
-
-
-
-
746900
Fesko
Expanding the threonine aldol ...
Aeromonas veronii
Appl. Microbiol. Biotechnol.
99
9651-9661
2015
-
-
1
-
-
-
-
3
-
-
-
-
-
1
-
-
1
-
-
-
-
-
8
-
-
-
-
3
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
1
-
-
-
-
8
-
-
-
-
3
-
-
-
-
-
-
-
-
3
3
748265
Wieteska
Improving thermal stability o ...
Thermotoga maritima
J. Biotechnol.
199
69-76
2015
-
1
1
-
11
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
2
1
1
-
1
-
-
-
-
1
-
-
-
-
1
1
1
-
11
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
713884
Dueckers
Threonine aldolases-screening, ...
Clostridium pasteurianum, Escherichia coli, Pseudomonas aeruginosa, Pseudomonas sp., Pseudomonas sp. NCIMB10558
Appl. Microbiol. Biotechnol.
88
409-424
2010
-
-
-
-
-
-
-
4
-
-
-
-
-
5
-
-
2
-
-
-
2
-
8
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
-
-
-
-
-
2
-
-
2
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5203
Pagani
DL-Allothreonine and L-threoni ...
Rattus norvegicus
Biochem. Soc. Trans.
19
346S
1991
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
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-
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-
-
-
-
-
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-
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2
-
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5191
Stoecklein
Evidence for L-threonine cleav ...
Clostridium pasteurianum
Biochem. J.
232
621-622
1985
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
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1
-
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1
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1
2
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1
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2
2
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1
-
-
-
-
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1
-
-
-
-
-
-
-
-
-
5199
Dainty
Purification and properties of ...
Clostridium pasteurianum
Biochem. J.
117
585-592
1970
-
-
-
-
-
-
4
1
-
-
-
-
-
1
-
-
1
-
-
-
-
1
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
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-
-
4
-
1
-
-
-
-
-
-
-
1
-
-
-
1
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
5200
Riario-Sforza
Threonine aldolase and allothr ...
Rattus norvegicus
Eur. J. Biochem.
8
88-92
1969
-
-
-
-
-
-
-
-
-
-
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1
-
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1
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1
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1
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1
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-
-
-
715411
Karasek
Greenberg, D.M.: Studies on th ...
Ovis aries
J. Biol. Chem.
227
191-205
1957
-
-
-
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3
-
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1
-
-
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1
-
-
1
-
-
1
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
1
-
-
-
-
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1
-
1
-
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1
-
-
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1
1
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