BRENDA - Enzyme Database show
show all sequences of 4.1.2.5

Threonine aldolases-screening, properties and applications in the synthesis of non-proteinogenic beta-hydroxy-alpha-amino acids

Dueckers, N.; Baer, K.; Simon, S.; Groeger, H.; Hummel, W.; Appl. Microbiol. Biotechnol. 88, 409-424 (2010)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.19
-
L-allo-threonine
pH and temperature not specified in the publication
Escherichia coli
10
-
L-threonine
pH and temperature not specified in the publication
Escherichia coli
14.6
-
L-allo-threonine
pH and temperature not specified in the publication
Pseudomonas sp.
14.7
-
L-threonine
pH and temperature not specified in the publication
Pseudomonas sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Clostridium pasteurianum
-
-
-
Escherichia coli
-
-
-
Pseudomonas aeruginosa
-
-
-
Pseudomonas sp.
-
-
-
Pseudomonas sp. NCIMB10558
-
-
-
Purification (Commentary)
Commentary
Organism
DEAE-cellulose column chromatography
Clostridium pasteurianum
DEAE-Toyopearl column chromatography
Pseudomonas sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
additional information
-
the crude extract has a specific activity of 0.02 units/mg, the purified enzyme has a specific activity of 0.32 units/mg, pH and temperature not specified in the publication
Clostridium pasteurianum
additional information
-
specific activity of 31 units/mg, using L-threonine as substrate, pH and temperature not specified in the publication
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-allo-threonine
-
713884
Escherichia coli
?
-
-
-
?
L-allo-threonine
-
713884
Pseudomonas sp.
?
-
-
-
?
L-allo-threonine
-
713884
Pseudomonas sp. NCIMB10558
?
-
-
-
?
L-threonine
L-TA preferably accepts L-threonine as substrate
713884
Escherichia coli
glycine + acetaldehyde
-
-
-
?
L-threonine
L-TA preferably accepts L-threonine as substrate
713884
Pseudomonas aeruginosa
glycine + acetaldehyde
-
-
-
?
L-threonine
L-TA preferably accepts L-threonine as substrate
713884
Pseudomonas sp.
glycine + acetaldehyde
-
-
-
?
L-threonine
L-TA preferably accepts L-threonine as substrate
713884
Clostridium pasteurianum
glycine + acetaldehyde
-
-
-
?
L-threonine
L-TA preferably accepts L-threonine as substrate
713884
Pseudomonas sp. NCIMB10558
glycine + acetaldehyde
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
dependent on
Clostridium pasteurianum
pyridoxal 5'-phosphate
dependent on
Escherichia coli
pyridoxal 5'-phosphate
dependent on
Pseudomonas aeruginosa
pyridoxal 5'-phosphate
dependent on
Pseudomonas sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
dependent on
Clostridium pasteurianum
pyridoxal 5'-phosphate
dependent on
Escherichia coli
pyridoxal 5'-phosphate
dependent on
Pseudomonas aeruginosa
pyridoxal 5'-phosphate
dependent on
Pseudomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.19
-
L-allo-threonine
pH and temperature not specified in the publication
Escherichia coli
10
-
L-threonine
pH and temperature not specified in the publication
Escherichia coli
14.6
-
L-allo-threonine
pH and temperature not specified in the publication
Pseudomonas sp.
14.7
-
L-threonine
pH and temperature not specified in the publication
Pseudomonas sp.
Purification (Commentary) (protein specific)
Commentary
Organism
DEAE-cellulose column chromatography
Clostridium pasteurianum
DEAE-Toyopearl column chromatography
Pseudomonas sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
additional information
-
the crude extract has a specific activity of 0.02 units/mg, the purified enzyme has a specific activity of 0.32 units/mg, pH and temperature not specified in the publication
Clostridium pasteurianum
additional information
-
specific activity of 31 units/mg, using L-threonine as substrate, pH and temperature not specified in the publication
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-allo-threonine
-
713884
Escherichia coli
?
-
-
-
?
L-allo-threonine
-
713884
Pseudomonas sp.
?
-
-
-
?
L-allo-threonine
-
713884
Pseudomonas sp. NCIMB10558
?
-
-
-
?
L-threonine
L-TA preferably accepts L-threonine as substrate
713884
Escherichia coli
glycine + acetaldehyde
-
-
-
?
L-threonine
L-TA preferably accepts L-threonine as substrate
713884
Pseudomonas aeruginosa
glycine + acetaldehyde
-
-
-
?
L-threonine
L-TA preferably accepts L-threonine as substrate
713884
Pseudomonas sp.
glycine + acetaldehyde
-
-
-
?
L-threonine
L-TA preferably accepts L-threonine as substrate
713884
Clostridium pasteurianum
glycine + acetaldehyde
-
-
-
?
L-threonine
L-TA preferably accepts L-threonine as substrate
713884
Pseudomonas sp. NCIMB10558
glycine + acetaldehyde
-
-
-
?
Other publictions for EC 4.1.2.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746894
Bulut
Development of a growth-depen ...
Pseudomonas putida
Appl. Microbiol. Biotechnol.
99
5875-5883
2015
-
1
-
-
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1
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1
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1
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1
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-
-
-
-
746900
Fesko
Expanding the threonine aldol ...
Aeromonas veronii
Appl. Microbiol. Biotechnol.
99
9651-9661
2015
-
-
1
-
-
-
-
3
-
-
-
-
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1
-
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1
-
-
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8
-
-
-
-
3
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
3
-
-
-
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-
-
-
1
-
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-
8
-
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3
-
-
-
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3
3
748265
Wieteska
Improving thermal stability o ...
Thermotoga maritima
J. Biotechnol.
199
69-76
2015
-
1
1
-
11
-
-
-
-
-
1
-
-
2
-
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2
1
1
-
1
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1
-
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-
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1
1
1
-
11
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
713884
Dueckers
Threonine aldolases-screening, ...
Clostridium pasteurianum, Escherichia coli, Pseudomonas aeruginosa, Pseudomonas sp., Pseudomonas sp. NCIMB10558
Appl. Microbiol. Biotechnol.
88
409-424
2010
-
-
-
-
-
-
-
4
-
-
-
-
-
5
-
-
2
-
-
-
2
-
8
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
-
-
-
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-
2
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2
-
8
-
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-
-
-
-
-
-
-
-
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-
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-
5203
Pagani
DL-Allothreonine and L-threoni ...
Rattus norvegicus
Biochem. Soc. Trans.
19
346S
1991
-
-
-
-
-
-
-
-
-
-
-
-
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2
-
-
-
-
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2
-
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1
-
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-
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-
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2
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1
-
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-
-
-
-
-
-
-
-
-
5191
Stoecklein
Evidence for L-threonine cleav ...
Clostridium pasteurianum
Biochem. J.
232
621-622
1985
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
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1
-
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-
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1
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1
2
-
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1
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-
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2
2
-
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-
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1
-
-
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-
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1
-
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-
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-
5199
Dainty
Purification and properties of ...
Clostridium pasteurianum
Biochem. J.
117
585-592
1970
-
-
-
-
-
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4
1
-
-
-
-
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1
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1
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1
1
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1
1
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1
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1
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4
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1
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-
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-
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1
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1
1
-
-
-
-
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1
1
-
-
-
-
-
-
-
-
5200
Riario-Sforza
Threonine aldolase and allothr ...
Rattus norvegicus
Eur. J. Biochem.
8
88-92
1969
-
-
-
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1
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1
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1
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1
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715411
Karasek
Greenberg, D.M.: Studies on th ...
Ovis aries
J. Biol. Chem.
227
191-205
1957
-
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3
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1
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1
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1
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1
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1
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1
1
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1
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1
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3
-
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1
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1
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1
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1
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1
1
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