Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | activity is decreased to 20% of the original by treatment with cysteine plus mercaptoethanol. Most of the loss is regained on incubation with pyridoxal 5'-phosphate | Clostridium pasteurianum | |
cysteine | activity is decreased to 20% of the original by treatment with cysteine plus mercaptoethanol. Most of the loss is regained on incubation with pyridoxal 5'-phosphate | Clostridium pasteurianum | |
hydroxylamine | 1 mM, complete inhibition | Clostridium pasteurianum | |
Semicarbazide | 1 mM, complete inhibition | Clostridium pasteurianum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.42 | - |
L-threonine | pH 7.0, 30°C | Clostridium pasteurianum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium pasteurianum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Clostridium pasteurianum |
Storage Stability | Organism |
---|---|
0-5°C, activity slowly decreases to 50% of the original activity after 10 days | Clostridium pasteurianum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-threonine | the enzyme is highly specific for L-threonine, no activity with D-threonine, no activity with DL-allo-threonine | Clostridium pasteurianum | glycine + acetaldehyde | - |
r |
Synonyms | Comment | Organism |
---|---|---|
L-threonine acetaldehyde-lyase | - |
Clostridium pasteurianum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | - |
Clostridium pasteurianum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 7.8 | pH 6.0: about 80% of maximal activity, pH 7.8: about 80% of maximal activity | Clostridium pasteurianum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Clostridium pasteurianum |