Protein Variants | Comment | Organism |
---|---|---|
D168N | mutation reduces the specific activity, which is partially restored by the addition of excess pyridoxal 5'-phosphate to the reaction mixture. The mutant enzyme is able to catalyse the aldol synthesis of L-beta-phenylserine from benzaldehyde and glycine with yields similar to the wild-type enzyme | Aeromonas jandaei |
D168S | mutation reduces the specific activity, which is partially restored by the addition of excess pyridoxal 5'-phosphate to the reaction mixture. The mutant enzyme is able to catalyse the aldol synthesis of L-beta-phenylserine from benzaldehyde and glycine with yields similar to the wild-type enzyme | Aeromonas jandaei |
D168T | mutation reduces the specific activity, which is partially restored by the addition of excess pyridoxal 5'-phosphate to the reaction mixture. The mutant enzyme is able to catalyse the aldol synthesis of L-beta-phenylserine from benzaldehyde and glycine with yields similar to the wild-type enzyme | Aeromonas jandaei |
D168V | mutation reduces the specific activity, which is partially restored by the addition of excess pyridoxal 5'-phosphate to the reaction mixture. The mutant enzyme is able to catalyse the aldol synthesis of L-beta-phenylserine from benzaldehyde and glycine with yields similar to the wild-type enzyme | Aeromonas jandaei |
K199R | mutation reduces the specific activity of the enzyme by 5000fold, which can be partially restored by adding an excess of pyridoxal 5'-phosphate to the reaction medium | Aeromonas jandaei |
R171F | loss of specific activity by more than 2000fold. pyridoxal 5'-phosphate-glycine complex is not obtained for the mutant. The mutant is not active in the aldol condensation reaction to produce L-beta-phenylserine | Aeromonas jandaei |
R231A | the mutant enzyme racemizes L-alanine to D-alanine, whereas the wild-type enzyme is not active towards L-alanine | Aeromonas jandaei |
R313H | catalytic activity of the mutant in the retro-aldol cleavage of L-allo-threonine is decreased by 200fold mainly due to increase in Km. Mutation hinders the formation of the quinonoid complex to stabilize the carbanion after initial bond cleavage. L-beta-Phenylserine product in the aldol condensation is obtained with the conversion rate similar to the wild-type enzyme | Aeromonas jandaei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme G200S | Aeromonas jandaei | |
0.5 | - |
L-allo-threonine | pH 8.0, 25°C, wild-type enzyme | Aeromonas jandaei | |
0.5 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme C196T | Aeromonas jandaei | |
0.8 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme N64I | Aeromonas jandaei | |
1 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme N64E | Aeromonas jandaei | |
1.2 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme D168S | Aeromonas jandaei | |
1.2 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme E138A | Aeromonas jandaei | |
1.3 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme D168V | Aeromonas jandaei | |
2.1 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme G200S | Aeromonas jandaei | |
2.3 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme E138A | Aeromonas jandaei | |
2.6 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme D168N | Aeromonas jandaei | |
2.7 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme D168V | Aeromonas jandaei | |
2.7 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme K199R | Aeromonas jandaei | |
3.7 | - |
L-beta-Phenylserine | pH 8.0, 25°C, wild-type enzyme | Aeromonas jandaei | |
3.7 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme C196T | Aeromonas jandaei | |
3.8 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme H85Y | Aeromonas jandaei | |
3.8 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme N64I | Aeromonas jandaei | |
4.1 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme D168S | Aeromonas jandaei | |
4.1 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme R231A | Aeromonas jandaei | |
4.6 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme H85F | Aeromonas jandaei | |
4.8 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme S198H | Aeromonas jandaei | |
5.2 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme R313H | Aeromonas jandaei | |
5.7 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme N64E | Aeromonas jandaei | |
5.7 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme S198H | Aeromonas jandaei | |
7 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme R171F | Aeromonas jandaei | |
7.5 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme R231A | Aeromonas jandaei | |
11.5 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme H85F | Aeromonas jandaei | |
14.8 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme H85Y | Aeromonas jandaei | |
17 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme R171Q | Aeromonas jandaei | |
18 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme R171F | Aeromonas jandaei | |
28 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme R313H | Aeromonas jandaei | |
69 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme R171Q | Aeromonas jandaei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeromonas jandaei | O07051 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycine + acetaldehyde | - |
Aeromonas jandaei | L-allo-threonine | - |
r | |
glycine + benzyladehyde | - |
Aeromonas jandaei | L-beta-phenylserine | - |
r | |
L-allo-threonine | - |
Aeromonas jandaei | glycine + acetaldehyde | - |
r | |
L-beta-phenylserine | - |
Aeromonas jandaei | glycine + benzyladehyde | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme H85Y | Aeromonas jandaei | |
0.02 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme D168V | Aeromonas jandaei | |
0.05 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme D168N | Aeromonas jandaei | |
0.05 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme H85F | Aeromonas jandaei | |
0.08 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme K199R | Aeromonas jandaei | |
0.08 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme N64E | Aeromonas jandaei | |
0.17 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme R171F | Aeromonas jandaei | |
0.2 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme H85Y | Aeromonas jandaei | |
0.2 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme N64I | Aeromonas jandaei | |
0.2 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme R171Q | Aeromonas jandaei | |
0.23 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme R171F | Aeromonas jandaei | |
0.3 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme H85F | Aeromonas jandaei | |
0.3 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme N64E | Aeromonas jandaei | |
0.32 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme R313H | Aeromonas jandaei | |
0.42 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme D168S | Aeromonas jandaei | |
0.47 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme R171Q | Aeromonas jandaei | |
0.6 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme D168V | Aeromonas jandaei | |
0.7 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme R231A | Aeromonas jandaei | |
0.83 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme D168S | Aeromonas jandaei | |
1 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme N64I | Aeromonas jandaei | |
1.9 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme E138A | Aeromonas jandaei | |
2.3 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme R231A | Aeromonas jandaei | |
2.3 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme R313H | Aeromonas jandaei | |
3.9 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme E138A | Aeromonas jandaei | |
4.3 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme S198H | Aeromonas jandaei | |
5.1 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme S198H | Aeromonas jandaei | |
8.67 | - |
L-allo-threonine | pH 8.0, 25°C, wild-type enzyme | Aeromonas jandaei | |
13.3 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme C196T | Aeromonas jandaei | |
13.6 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme G200S | Aeromonas jandaei | |
60.5 | - |
L-beta-Phenylserine | pH 8.0, 25°C, wild-type enzyme | Aeromonas jandaei | |
74.4 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme G200S | Aeromonas jandaei | |
84 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme C196T | Aeromonas jandaei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on | Aeromonas jandaei |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0007 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme H85Y | Aeromonas jandaei | |
0.0045 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme H85F | Aeromonas jandaei | |
0.0068 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme R171Q | Aeromonas jandaei | |
0.01 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme R171F | Aeromonas jandaei | |
0.011 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme R171Q | Aeromonas jandaei | |
0.02 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme D168N | Aeromonas jandaei | |
0.024 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme R171F | Aeromonas jandaei | |
0.03 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme K199R | Aeromonas jandaei | |
0.05 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme H85Y | Aeromonas jandaei | |
0.06 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme N64E | Aeromonas jandaei | |
0.062 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme R313H | Aeromonas jandaei | |
0.063 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme H85F | Aeromonas jandaei | |
0.067 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme D168V | Aeromonas jandaei | |
0.08 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme N64E | Aeromonas jandaei | |
0.08 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme R313H | Aeromonas jandaei | |
0.09 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme R231A | Aeromonas jandaei | |
0.2 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme D168S | Aeromonas jandaei | |
0.3 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme N64I | Aeromonas jandaei | |
0.3 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme N64I | Aeromonas jandaei | |
0.35 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme D168S | Aeromonas jandaei | |
0.4 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme D168V | Aeromonas jandaei | |
0.6 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme R231A | Aeromonas jandaei | |
0.9 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme S198H | Aeromonas jandaei | |
0.9 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme S198H | Aeromonas jandaei | |
1.6 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme E138A | Aeromonas jandaei | |
1.7 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme E138A | Aeromonas jandaei | |
16 | - |
L-beta-Phenylserine | pH 8.0, 25°C, wild-type enzyme | Aeromonas jandaei | |
17 | - |
L-allo-threonine | pH 8.0, 25°C, wild-type enzyme | Aeromonas jandaei | |
22 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme C196T | Aeromonas jandaei | |
26 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme C196T | Aeromonas jandaei | |
35 | - |
L-beta-Phenylserine | pH 8.0, 25°C, mutant enzyme G200S | Aeromonas jandaei | |
61 | - |
L-allo-threonine | pH 8.0, 25°C, mutant enzyme G200S | Aeromonas jandaei |