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Literature summary for 4.1.2.49 extracted from

  • Qin, H.M.; Imai, F.L.; Miyakawa, T.; Kataoka, M.; Kitamura, N.; Urano, N.; Mori, K.; Kawabata, H.; Okai, M.; Ohtsuka, J.; Hou, F.; Nagata, K.; Shimizu, S.; Tanokura, M.
    L-allo-threonine aldolase with an H128Y/S292R mutation from Aeromonas jandaei DK-39 reveals the structural basis of changes in substrate stereoselectivity (2014), Acta Crystallogr. Sect. D, 70, 1695-1703 .
    View publication on PubMed
Search for more literature for 4.1.2.49

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rosetta(DE3) Aeromonas jandaei

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting-drop vapour-diffusion method, crystal structures of wild-type enzyme and its mutant H128Y/S292R are determined at 2.59 and 2.50 A resolution Aeromonas jandaei

Protein Variants

Protein Variants Comment Organism
H128Y 8.4fold increase in activity towards L-threonine and a 2.0fold increase towards L-allo-threonine compared with the wild-type enzyme Aeromonas jandaei
H128Y/S292R 3fold and 322fold increased kcat/Km values towards towards L-allo-threonine and L-threonine compared with the wild-type enzyme Aeromonas jandaei
S292R improved activity towards L-allo-threonine and L-threonine Aeromonas jandaei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.386
-
 L-allo-threonine pH 8.0, 30°C, mutant enzyme H128Y/S292R Aeromonas jandaei
0.402
-
 L-allo-threonine pH 8.0, 30°C, mutant enzyme H128Y Aeromonas jandaei
0.444
-
 L-allo-threonine pH 8.0, 30°C, mutant enzyme S292R Aeromonas jandaei
0.513
-
 L-allo-threonine pH 8.0, 30°C, wild-type enzyme Aeromonas jandaei
3.27
-
 L-threonine pH 8.0, 30°C, mutant enzyme H128Y/S292R Aeromonas jandaei
4.48
-
 L-threonine pH 8.0, 30°C, mutant enzyme H128Y Aeromonas jandaei
31.6
-
 L-threonine pH 8.0, 30°C, wild-type enzyme Aeromonas jandaei
32.7
-
 L-threonine pH 8.0, 30°C, mutant enzyme S292R Aeromonas jandaei

Organism

Organism UniProt Comment Textmining
Aeromonas jandaei O07051
-
-
Aeromonas jandaei DK-39 O07051
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aeromonas jandaei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-allo-threonine
-
 Aeromonas jandaei glycine + acetaldehyde
-
 r
L-allo-threonine
-
 Aeromonas jandaei DK-39 glycine + acetaldehyde
-
 r
L-threonine low activity Aeromonas jandaei glycine + acetaldehyde
-
 r
L-threonine low activity Aeromonas jandaei DK-39 glycine + acetaldehyde
-
 r

Synonyms

Synonyms Comment Organism
LATA
-
 Aeromonas jandaei
ltaA
-
 Aeromonas jandaei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.641
-
 L-threonine pH 8.0, 30°C, wild-type enzyme Aeromonas jandaei
0.913
-
 L-threonine pH 8.0, 30°C, mutant enzyme S292R Aeromonas jandaei
1.81
-
 L-threonine pH 8.0, 30°C, mutant enzyme H128Y Aeromonas jandaei
2.14
-
 L-threonine pH 8.0, 30°C, mutant enzyme H128Y/S292R Aeromonas jandaei
13.8
-
 L-allo-threonine pH 8.0, 30°C, wild-type enzyme Aeromonas jandaei
16.5
-
 L-allo-threonine pH 8.0, 30°C, mutant enzyme H128Y Aeromonas jandaei
18.3
-
 L-allo-threonine pH 8.0, 30°C, mutant enzyme S292R Aeromonas jandaei
22
-
 L-allo-threonine pH 8.0, 30°C, mutant enzyme H128Y/S292R Aeromonas jandaei

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate dependent on Aeromonas jandaei

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00203
-
 L-threonine pH 8.0, 30°C, wild-type enzyme Aeromonas jandaei
0.0279
-
 L-threonine pH 8.0, 30°C, mutant enzyme S292R Aeromonas jandaei
0.404
-
 L-threonine pH 8.0, 30°C, mutant enzyme H128Y Aeromonas jandaei
0.654
-
 L-threonine pH 8.0, 30°C, mutant enzyme H128Y/S292R Aeromonas jandaei
26.9
-
 L-allo-threonine pH 8.0, 30°C, wild-type enzyme Aeromonas jandaei
41
-
 L-allo-threonine pH 8.0, 30°C, mutant enzyme H128Y Aeromonas jandaei
41.2
-
 L-allo-threonine pH 8.0, 30°C, mutant enzyme S292R Aeromonas jandaei
83.3
-
 L-allo-threonine pH 8.0, 30°C, mutant enzyme H128Y/S292R Aeromonas jandaei