Application | Comment | Organism |
---|---|---|
pharmacology | biotechnological potential for the syntheses of pharmaceutically relevant drug molecules because of the stereospecificity | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion, low-pH crystal structure of the enzyme at 2.1 A resolution, with a noncovalently bound uncleaved L-serine substrate, and a pyridoxal 5'-phosphate cofactor bound as an internal aldimine. This structure contrasts with other Escherichia coli L-threonine aldolase structures obtained at physiological pH that show products or substrates bound as pyridoxal 5'-phosphate-external aldimines. The non-productive binding at low-pH is due to an unusual substrate serine binding orientation in which the alpha-amino group and carboxylate group are in the wrong positions (relative to the active site residues) as a result of protonation of the alpha-amino group of the serine, as well as the active site histidines, His83 and His126. Protonation of these residues prevent the characteristic nucleophilic attack of the alpha-amino group of substrate serine on C4' of pyridoxal 5'-phosphate to form the external aldimine. At low pH the change in charge distribution at the active site can result in substrates binding in a non-productive orientation | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycine + acetaldehyde | - |
Escherichia coli | L-threonine | - |
r | |
L-threonine | - |
Escherichia coli | glycine + acetaldehyde | - |
r |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
metabolism | reversible cleavage of L-3-hydroxy-alpha-amino acids to glycine and the corresponding aldehydes | Escherichia coli |