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Literature summary for 4.1.2.47 extracted from

  • Guterl, J.K.
    Andexer, J.N.; Sehl, T.; von Langermann, J.; Frindi-Wosch, I.; Rosenkranz, T.; Fitter, J.; Gruber, K.; Kragl, U.; Eggert, T.; Pohl, M.: Uneven twins: comparison of two enantiocomplementary hydroxynitrile lyases with alpha/beta-hydrolase fold (2009), J. Biotechnol., 141, 166-173.
    View publication on PubMed
Search for more literature for 4.1.2.47

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Manihot esculenta
expression in Escherichia coli Manihot esculenta

Protein Variants

Protein Variants Comment Organism
C81A the mutant is also inhibited by acetate Manihot esculenta

General Stability

General Stability Organism
HNL is stabilized by entrapment in aggregates of Escherichia coli cell proteins Manihot esculenta
the enzyme is stabilized in crude cell extract Manihot esculenta

Inhibitors

Inhibitors Comment Organism Structure
acetate
-
 Hevea brasiliensis
acetate
-
 Manihot esculenta
additional information no inhibition by acetate Manihot esculenta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1
-
 (S)-mandelonitrile pH 7.5 Manihot esculenta
4.1
-
 (S)-mandelonitrile citrate buffer (50 mM, pH 4.0), at 25°C Manihot esculenta
5.9
-
 benzaldehyde pH 7.5 Manihot esculenta
5.9
-
 benzaldehyde citrate buffer (50 mM, pH 4.0), at 25°C Manihot esculenta
179
-
 cyanide pH 7.5 Manihot esculenta
179
-
 cyanide citrate buffer (50 mM, pH 4.0), at 25°C Manihot esculenta

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29200
-
 4 * 29200, in solution, calculated from amino acid sequence Hevea brasiliensis
29200
-
 4 * 29200, in solution, calculated from amino acid sequence Manihot esculenta
124000
-
 gel filtration Manihot esculenta

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Acetone cyanhydrin Manihot esculenta
-
 Cyanide + acetone
-
 ?

Organism

Organism UniProt Comment Textmining
Hevea brasiliensis
-
-
-
Manihot esculenta
-
-
-
Manihot esculenta P52705
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography Manihot esculenta
recombinant enzyme Manihot esculenta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-mandelonitrile
-
 Hevea brasiliensis cyanide + benzaldehyde
-
 r
(S)-mandelonitrile
-
 Manihot esculenta cyanide + benzaldehyde
-
 r
Acetone cyanhydrin
-
 Manihot esculenta Cyanide + acetone
-
 ?
cyanide + benzaldehyde
-
 Manihot esculenta (S)-mandelonitrile
-
 r
cyclohexanone cyanohydrin
-
 Manihot esculenta ?
-
 ?
lactonitrile poor substrate Manihot esculenta ?
-
 ?
m-phenoxybenzaldehyde cyanohydrin 2-phenoxybenzaldehyde cyanohydrin is converted with lower activity than (S)-mandelonitrile and cyclohexanone cyanohydrile Manihot esculenta m-phenoxybenzaldehyde + cyanide
-
 ?
p-hydroxymandelonitrile phydroxymandelonitrile is converted with lower activity than (S)-mandelonitrile and cyclohexanone cyanohydrile Manihot esculenta ?
-
 ?
propionaldehyde cyanohydrin poor substrate Manihot esculenta propionaldehyde + cyanide
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 29200, in solution, calculated from amino acid sequence Hevea brasiliensis
homotetramer 4 * 29200, in solution, calculated from amino acid sequence Manihot esculenta
tetramer the enzyme forms dimers in solution Manihot esculenta

Synonyms

Synonyms Comment Organism
MeHNL
-
 Manihot esculenta
S-HnL
-
 Hevea brasiliensis
S-HnL
-
 Manihot esculenta
S-hydroxynitrile lyase
-
 Manihot esculenta
S-selective HnL
-
 Hevea brasiliensis
S-selective HnL
-
 Manihot esculenta

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Manihot esculenta

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
 60 the enzyme is stable and exhibits more than 96 h half-life time between 0 and 20°C, the enzyme shows more than 48 h half-life time at 30°C, 64 h half-life time at 37°C, 2.7 h half-life time at 50°C, and 0.5 h half-life time at 60°C Manihot esculenta
20
-
 half-life time: more than 96 h Manihot esculenta
37
-
 half-life time: 64 h Manihot esculenta
40 50 the enzyme shows a half-life time of 4–11 h at 40°C. At 50°C a rapid inactivation is observed Hevea brasiliensis
50
-
 half-life time: 2.7 h Manihot esculenta
60
-
 half-life time: 30 min Manihot esculenta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
-
 Hevea brasiliensis
5.8
-
-
 Manihot esculenta

pH Range

pH Minimum pH Maximum Comment Organism
4 5.8 pH 4: about 40% of maximal activity, pH 5.8: optimum Manihot esculenta

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 half-life time: 2 h, the deactivation of the enzyme at slightly acidic pH is a result of pronounced structural unfolding Manihot esculenta
4
-
 the enzyme shows a half-life time of ca. 4 h at pH 5.0 Hevea brasiliensis
4
-
 the enzyme shows still 40% of its maximal activity at pH 4.0, while its activity is drastically reduced below pH 4.0 Manihot esculenta

pI Value

Organism Comment pI Value Maximum pI Value
Hevea brasiliensis isoelectric focusing
-
 5.3
Manihot esculenta isoelectric focusing
-
 5.3