Literature summary for 4.1.2.47 extracted from

von Langermann, J.; Guterl, J.K.; Pohl, M.; Wajant, H.; Kragl, U.
Hydroxynitrile lyase catalyzed cyanohydrin synthesis at high pH-values (2008), Bioprocess Biosyst. Eng., 31, 155-161.

Search for more literature for 4.1.2.47

Application

Application	Comment	Organism
synthesis	synthesis of enantiopure (S)-3-phenoxybenzaldehyde cyanohydrin (a useful intermediate in the pyrethroid synthesis) by applying a high pH two-phase system to reduce nonenzymatic reaction	Manihot esculenta

Protein Variants

Protein Variants	Comment	Organism
W128A	although the variant W128A shows a higher activity with respect to (S)-3-phenoxy-benzaldehyde cyanohydrin, the enantioselectivity is reduced to 85%, compared to 97% of wild-type enzyme	Manihot esculenta

Organism

Organism	UniProt	Comment	Textmining
Manihot esculenta	P52705	-	-

Purification (Commentary)

Purification (Comment)	Organism
wild-type and W128A mutant partially purified	Manihot esculenta

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(S)-mandelonitrile	-	Manihot esculenta	cyanide + benzaldehyde	-	?
2-hydroxy-2-methylpropanenitrile	-	Manihot esculenta	cyanide + acetone	-	?
cyanide + 3-phenoxybenzaldehyde	reaction in a high-pH two-phase system	Manihot esculenta	(S)-3-phenoxybenzaldehyde cyanohydrin	97% enantiomeric excess	r

Synonyms

Synonyms	Comment	Organism
(S)-selective hydroxynitrile lyase	-	Manihot esculenta
MeHNL	-	Manihot esculenta

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.5	-	cleavage of mandelonitrile, cleavage of 2-hydroxy-2-methylpropanenitrile	Manihot esculenta
8	-	synthesis of (S)-3-phenoxybenzaldehyde cyanohydrin	Manihot esculenta

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
3	-	half-life: 47 h	Manihot esculenta
4	-	half-life: 84 h	Manihot esculenta
5	8	half-life: above 100 h	Manihot esculenta

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Release 2023.1 (January 2023)