Literature summary for 4.1.2.47 extracted from

Rustler, S.; Motejadded, H.; Altenbuchner, J.; Stolz, A.
Simultaneous expression of an arylacetonitrilase from Pseudomonas fluorescens and a (S)-oxynitrilase from Manihot esculenta in Pichia pastoris for the synthesis of (S)-mandelic acid (2008), Appl. Microbiol. Biotechnol., 80, 87-97.

Application

Application	Comment	Organism
synthesis	recombinant Pichia pastoris strains are constructed which simultaneously express the (S)-oxynitrilase of Manihot esculenta and the arylacetonitrilase of Pseudomonas fluorescens EBC191 each under the control of individual AOX1 promoters in order to obtain a whole cell catalyst for the synthesis of (S)-mandelic acid from benzaldehyde and cyanideproduction of optically active cyanohydrin compounds	Manihot esculenta

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant Pichia pastoris strains are constructed which simultaneously expressed the (S)-oxynitrilase of Manihot esculenta and the arylacetonitrilase of Pseudomonas fluorescens EBC191 each under the control of individual AOX1 promoters in order to obtain a whole cell catalyst for the synthesis of (S)-mandelic acid from benzaldehyde and cyanide production of optically active cyanohydrin compounds	Manihot esculenta

Cloned (Comment)

Organism

recombinant Pichia pastoris strains are constructed which simultaneously expressed the (S)-oxynitrilase of Manihot esculenta and the arylacetonitrilase of Pseudomonas fluorescens EBC191 each under the control of individual AOX1 promoters in order to obtain a whole cell catalyst for the synthesis of (S)-mandelic acid from benzaldehyde and cyanide production of optically active cyanohydrin compounds

Manihot esculenta

Organism

Organism	UniProt	Comment	Textmining
Manihot esculenta	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cyanide + benzaldehyde	-	Manihot esculenta	(S)-mandelonitrile	-	?

Synonyms

Synonyms	Comment	Organism
(S)-Oxynitrilase	-	Manihot esculenta

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	-	-	Manihot esculenta

pH Range

pH Minimum	pH Maximum	Comment	Organism
3	7	-	Manihot esculenta

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Release 2023.1 (January 2023)