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Literature summary for 4.1.2.47 extracted from

  • Bauer, M.; Griengl, H.; Steiner, W.
    Parameters influencing stability and activity of a S-hydroxynitrile lyase from Hevea brasiliensis in two-phase systems (1999), Enzyme Microb. Technol., 24, 514-522.
No PubMed abstract available
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General Stability

General Stability Organism
half-life at pH 5 and 20°C is halved at a concentration of 2 mM benzaldehyde and 30 mM HCN, stability decreases drastically with increasing concentrations of benzaldehyde Hevea brasiliensis
half-life increases linearly with enzyme concentration in the aqueous buffer as well as in a two-phase system of buffer and dibutyl ether Hevea brasiliensis
influence of different organic solvents and solvent mixtures on the stability in two-phase systems of aqueous buffer and organic solvent Hevea brasiliensis
the enzyme shows especially high stability in mixtures of methyl-tert-butylether and hexane, 40:60 and 25:75 Hevea brasiliensis
the use of solvent mixtures with methyl-tert-butylether leads to better stability than the use of pure solvents Hevea brasiliensis

Inhibitors

Inhibitors Comment Organism Structure
mandelonitrile
-
 Hevea brasiliensis

Organic Solvent Stability

Organic Solvent Comment Organism
dibutyl ether inactivates within a few min Hevea brasiliensis
Ethyl acetate inactivates within a few min Hevea brasiliensis
hexane inactivates within a few min, the use of solvent mixtures with methyl-tert-butylether leads to better stability than the use of pure solvents the enzyme shows especially high stability in mixtures of methyl-tert-butylether and hexane, 40:60 and 25:75 Hevea brasiliensis
methyl-tert-butylether the use of solvent mixtures with methyl-tert-butylether leads to better stability than the use of pure solvents the enzyme shows especially high stability in mixtures of methyl-tert-butylether and hexane, 40:60 and 25:75 Hevea brasiliensis

Organism

Organism UniProt Comment Textmining
Hevea brasiliensis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
rac-mandelonitrile
-
 Hevea brasiliensis HCN + benzaldehyde
-
 ?

Synonyms

Synonyms Comment Organism
(S)-Hydroxynitrile lyase
-
 Hevea brasiliensis
HbHNL
-
 Hevea brasiliensis
HNL
-
 Hevea brasiliensis

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5
-
 below pH 4.5 enzyme is inactivated within a few min Hevea brasiliensis
5
-
 half-life in stirred solution is approximately 3.5 h Hevea brasiliensis