Literature summary for 4.1.2.47 extracted from

B�hler, H.; Effenberger, F.; F�rster, S.; Roos, J.; Wajant, H.
Substrate specificity of mutants of the hydroxynitrile lyase from Manihot esculenta (2003), ChemBioChem, 4, 211-216.

Protein Variants

Protein Variants	Comment	Organism
W128A	substitution of tryptophan128 by an alanine residue enlarges the entrance channel to the active site of MeHNL and thus facilitates access of sterically demanding substrates to the active site, increased conversion rate towards 3-phenoxybenzaldehyde, octan-3-one and heptan-3-one	Manihot esculenta
W128C	mutation increases the specific activity towards 4-hydroxymandelonitrile of the various MeHNL mutant compared to the wild-type enzyme	Manihot esculenta
W128L	mutation increases the specific activity towards 4-hydroxymandelonitrile of the various MeHNL mutant compared to the wild-type enzyme	Manihot esculenta
W128Y	mutation increases the specific activity towards 4-hydroxymandelonitrile of the various MeHNL mutant compared to the wild-type enzyme	Manihot esculenta

Organism

Organism	UniProt	Comment	Textmining
Manihot esculenta	P52705	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Manihot esculenta

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
cyanide + (2E)-3-(4-hydroxyphenyl)prop-2-enal	-	Manihot esculenta	(2S,3E)-2-hydroxy-4-(4-hydroxyphenyl)but-3-enenitrile	wild-type enzyme: 95% enantiomeric excess, 80% conversion rate	?
cyanide + (2E)-hex-2-enal	-	Manihot esculenta	(2S,3E)-2-hydroxyhept-3-enenitrile	wild-type enzyme: 97% enantiomeric excess, 58% conversion rate	?
cyanide + (4-hydroxyphenyl)acetaldehyde	-	Manihot esculenta	(2S)-2-hydroxy-3-(4-hydroxyphenyl)propanenitrile	wild-type enzyme: 96% enantiomeric excess, 88% conversion rate	?
cyanide + 1-phenylethanone	wild-type enzyme: 87% enantiomeric excess, 13% conversion rate	Manihot esculenta	(2S)-2-hydroxy-2-phenylpropanenitrile	-	?
cyanide + 1-phenylpropan-2-one	wild-type enzyme: 97% enantiomeric excess, 82% conversion rate	Manihot esculenta	(2S)-2-hydroxy-2-methyl-3-phenylpropanenitrile	-	?
cyanide + 2-bromobenzaldehyde	-	Manihot esculenta	(2S)-(2-bromophenyl)(hydroxy)ethanenitrile	wild-type enzyme: 96% enantiomeric excess, 96% conversion rate	?
cyanide + 2-chlorobenzaldehyde	-	Manihot esculenta	(2S)-(2-chlorophenyl)(hydroxy)ethanenitrile	wild-type enzyme: 98% enantiomeric excess, 96% conversion rate	?
cyanide + 2-hydroxybenzaldehyde	-	Manihot esculenta	(2S)-(2-hydroxyphenyl)(hydroxy)ethanenitrile	wild-type enzyme: 91% enantiomeric excess, 47% conversion rate	?
cyanide + 3-(4-hydroxyphenyl)propanal	-	Manihot esculenta	(2S)-2-hydroxy-4-(4-hydroxyphenyl)butanenitrile	wild-type enzyme: 67% enantiomeric excess, 90% conversion rate	?
cyanide + 3-hydroxybenzaldehyde	-	Manihot esculenta	(2S)-(3-hydroxyphenyl)(hydroxy)ethanenitrile	wild-type enzyme: 97% enantiomeric excess, 88% conversion rate	?
cyanide + 4-hydroxybenzaldehyde	-	Manihot esculenta	(2S)-(4-hydroxyphenyl)(hydroxy)ethanenitrile	wild-type enzyme: 94% enantiomeric excess, 51% conversion rate	?
cyanide + 4-methoxybenzaldehyde	-	Manihot esculenta	(2S)-(4-methoxyphenyl)(hydroxy)ethanenitrile	wild-type enzyme: 99% enantiomeric excess, 79% conversion rate	?
cyanide + 4-methylbenzaldehyde	-	Manihot esculenta	(2S)-(4-methylphenyl)(hydroxy)ethanenitrile	wild-type enzyme: 99% enantiomeric excess, 50% conversion rate	?
cyanide + 4-phenoxybenzaldehyde	-	Manihot esculenta	(2S)-(4-phenoxyphenyl)(hydroxy)ethanenitrile	wild-type enzyme: 96% enantiomeric excess, 47% conversion rate	?
cyanide + 4-phenylbutan-2-one	wild-type enzyme: 49% enantiomeric excess, 36% conversion rate	Manihot esculenta	(2S)-2-hydroxy-2-methyl-4-phenylbutanenitrile	-	?
cyanide + 6-methylhept-5-en-2-one	wild-type enzyme: 61% enantiomeric excess, 78% conversion rate	Manihot esculenta	(2S)-2-hydroxy-2,6-dimethylhept-5-enenitrile	-	?
cyanide + benzaldehyde	-	Manihot esculenta	(S)-mandelonitrile	wild-type enzyme: 99% enantiomeric excess, 97% conversion rate	?
cyanide + decanal	-	Manihot esculenta	(2S)-2-hydroxyundecanenitrile	wild-type enzyme: 78% enantiomeric excess, 65% conversion rate	?
cyanide + dodecanal	-	Manihot esculenta	(2S)-2-hydroxytridecanenitrile	wild-type enzyme: 71% enantiomeric excess, 80% conversion rate	?
cyanide + heptan-3-one	wild-type enzyme: 46% enantiomeric excess, 14% conversion rate	Manihot esculenta	(2S)-2-ethyl-2-hydroxyhexanenitrile	-	?
cyanide + nonanal	-	Manihot esculenta	(2S)-2-hydroxydecanenitrile	wild-type enzyme: 80% enantiomeric excess, 99% conversion rate	?
cyanide + octan-3-one	wild-type enzyme: 61% enantiomeric excess, 24% conversion rate	Manihot esculenta	(2S)-2-ethyl-2-hydroxyheptanenitrile	-	?
cyanide + octanal	-	Manihot esculenta	(2S)-2-hydroxynonanenitrile	wild-type enzyme: 79% enantiomeric excess, 96% conversion rate	?
cyanide + phenylacetaldehyde	-	Manihot esculenta	2-hydroxy-3-phenylpropanenitrile	wild-type enzyme: 98% enantiomeric excess, 99% conversion rate	?