BRENDA - Enzyme Database
show all sequences of 4.1.2.46

Hydroxynitrile lyases: biological sources and application as biocatalysts

Fechter, M.H.; Griengl, H.; Food Technol. Biotechnol. 42, 287-294 (2004)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
cloned into Pichia pastoris, expressed in Escherichia coli as an N-terminal hexa-histidine fusion protein
Linum usitatissimum
Organism
Organism
UniProt
Commentary
Textmining
Linum usitatissimum
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(R)-2-butanone-cyanhydrin
-
661809
Linum usitatissimum
HCN + butanone
-
-
-
?
additional information
no activity with aromatic substrates
661809
Linum usitatissimum
?
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
LuHNL
-
Linum usitatissimum
Cloned(Commentary) (protein specific)
Commentary
Organism
cloned into Pichia pastoris, expressed in Escherichia coli as an N-terminal hexa-histidine fusion protein
Linum usitatissimum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(R)-2-butanone-cyanhydrin
-
661809
Linum usitatissimum
HCN + butanone
-
-
-
?
additional information
no activity with aromatic substrates
661809
Linum usitatissimum
?
-
-
-
?
Other publictions for EC 4.1.2.46
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
728212
Liu
-
Asymmetric synthesis of silico ...
Prunus persica
J. Shenzhen Univ. Sci. Eng.
28
54-59
2011
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690307
Cabirol
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Linum usitatissimum hydroxynit ...
Linum usitatissimum
Adv. Synth. Catal.
350
2329-2338
2008
1
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706798
Roberge
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Synthesis of optically active ...
Linum usitatissimum
Tetrahedron Asymmetry
18
208-214
2007
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661809
Fechter
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Hydroxynitrile lyases: biologi ...
Linum usitatissimum
Food Technol. Biotechnol.
42
287-294
2004
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652954
de Gonzalo
-
(R)-Oxynitrilase-catalyzed tra ...
Linum usitatissimum
J. Mol. Catal. B
19-20
223-230
2002
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1
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705204
Breithaupt
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Cloning and expression of (R)- ...
Linum usitatissimum
J. Mol. Catal. B
6
315-332
1999
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706342
Trummler
-
Expression of the Zn2+-contain ...
Linum usitatissimum
Plant Sci.
139
19-27
1998
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1
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2
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1
2
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1
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11
1
1
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11
1
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704386
Trummler
Molecular cloning of acetone c ...
Linum usitatissimum
J. Biol. Chem.
272
4770-4774
1997
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706341
Wajant
-
Immunocytological localization ...
Linum usitatissimum
Plant Sci.
103
145-154
1994
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5143
Albrecht
Improved purification of an (R ...
Linum usitatissimum
Biotechnol. Appl. Biochem.
17
191-203
1993
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1
1
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2
2
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1
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18
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18
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5142
Xu
Purification and characterizat ...
Linum usitatissimum
Arch. Biochem. Biophys.
263
256-263
1988
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