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Literature summary for 4.1.2.46 extracted from
- Improved purification of an (R)-oxynitrilase from Linum usitatissimum (flax) and investigation of the substrate range (1993), Biotechnol. Appl. Biochem., 17, 191-203.
General Stability
| General Stability | Organism |
|---|---|
| immobilization on Eupergit (carrier consisting of macroporous beads) improves the stability considerably in the pH range below pH 5 | Linum usitatissimum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| benzaldehyde | leads to a complete and irreversible deactivation of the enzyme within 2 h incubation | Linum usitatissimum |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
2 * 43000, SDS-PAGE | Linum usitatissimum |
| 87000 | - |
gel filtration | Linum usitatissimum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cyanide + acetone | Linum usitatissimum | natural substrates for the (R)-oxynitrilase from Linum usitatissimum are acetone and butan-2-one, which are the building blocks of the cyanogenic glycosides in Linum, linamarin and lotaustralin, or linustatin and neolinustatin, respectively | 2-hydroxy-2-methylpropanenitrile | - |
? | |
| cyanide + butan-2-one | Linum usitatissimum | natural substrates for the (R)-oxynitrilase from Linum usitatissimum are acetone and butan-2-one, which are the building blocks of the cyanogen glycosides in Linum, linamarin and lotaustralin, or linustatin and neolinustatin, respectively | (2R)-butan-2-one cyanohydrin | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Linum usitatissimum | P93243 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Linum usitatissimum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Linum usitatissimum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 52.9 | - |
- |
Linum usitatissimum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cyanide + 2-methylcyclopentanone | - |
Linum usitatissimum | ? | - |
? | |
| cyanide + acetone | - |
Linum usitatissimum | 2-hydroxy-2-methylpropanenitrile | - |
? | |
| cyanide + acetone | natural substrates for the (R)-oxynitrilase from Linum usitatissimum are acetone and butan-2-one, which are the building blocks of the cyanogenic glycosides in Linum, linamarin and lotaustralin, or linustatin and neolinustatin, respectively | Linum usitatissimum | 2-hydroxy-2-methylpropanenitrile | - |
? | |
| cyanide + acetylcyclopropane | - |
Linum usitatissimum | ? | - |
? | |
| cyanide + butan-2-one | - |
Linum usitatissimum | (2R)-2-hydroxy-2-methylbutanenitrile | 77.2% enantiomeric excess | ? | |
| cyanide + butan-2-one | natural substrates for the (R)-oxynitrilase from Linum usitatissimum are acetone and butan-2-one, which are the building blocks of the cyanogen glycosides in Linum, linamarin and lotaustralin, or linustatin and neolinustatin, respectively | Linum usitatissimum | (2R)-butan-2-one cyanohydrin | - |
? | |
| cyanide + butyraldehyde | - |
Linum usitatissimum | (2R)-2-hydroxypentanenitrile | - |
? | |
| cyanide + chloroacetone | - |
Linum usitatissimum | (2R)-3-chloro-2-hydroxy-2-methylpropionitrile | - |
? | |
| cyanide + crotonaldehyde | - |
Linum usitatissimum | (2R)-2-hydroxy-3-pentenenitrile | - |
? | |
| cyanide + hexan-2-one | - |
Linum usitatissimum | (2R)-2-hydroxy-2-methylhexanenitrile | - |
? | |
| cyanide + hydroxyacetone | - |
Linum usitatissimum | (2R)-1,2-dihydroxy-2-methyl-propane-3-nitrile | - |
? | |
| cyanide + methyl vinyl ketone | - |
Linum usitatissimum | (2R)-2-hydroxy-2-methyl-3-butenenitrile | - |
? | |
| cyanide + pentan-2,4-dione | - |
Linum usitatissimum | ? | - |
? | |
| cyanide + pentan-2-one | - |
Linum usitatissimum | (2R)-2-hydroxy-2-methylpentanenitrile | - |
? | |
| cyanide + pinacolone | - |
Linum usitatissimum | (2R)-2-hydroxy-2,3,3-trimethylbutyronitrile | - |
? | |
| cyanide + pivalaldehyde | - |
Linum usitatissimum | (2R)-3,3-dimethyl-2-hydroxybutyronitrile | - |
? | |
| cyanide + propionaldehyde | - |
Linum usitatissimum | (2R)-2-hydroxybutyronitrile | - |
? | |
| cyanide + pyruvic acid ethyl ester | - |
Linum usitatissimum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 43000, SDS-PAGE | Linum usitatissimum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| (R)-Oxynitrilase | - |
Linum usitatissimum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Linum usitatissimum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Linum usitatissimum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.1 | 5.5 | pH 4.1: about 70% of maximal activity, pH 5.5: maximal activity | Linum usitatissimum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
half-life: 1 h | Linum usitatissimum |
| 5 | - |
immobilization on Eupergit (carrier consisting of macroporous beads) improves the stability considerably in the pH range below pH 5 | Linum usitatissimum |
| 6 | 11 | stable | Linum usitatissimum |
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