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Literature summary for 4.1.2.45 extracted from
- Characterization of the aldol condensation activity of the trans-o-hydroxybenzylidenepyruvate hydratase-aldolase (tHBP-HA) cloned from Pseudomonas fluorescens N3 (2011), Biochim. Biophys. Acta, 1814, 622-629.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli JM109 (pVLT33-ALD) and M15 (pREP4) cells | Pseudomonas fluorescens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.58 | - |
Salicylaldehyde | at pH 7.0, 30°C | Pseudomonas fluorescens |  |
| 8.98 | - |
benzaldehyde | at pH 7.0, 30°C | Pseudomonas fluorescens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
x * 38000, His-tagged recombinant enzyme, SDS-PAGE | Pseudomonas fluorescens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | - |
- |
- |
| Pseudomonas fluorescens N3 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Co2+ Talon metal affinity resin column chromatography | Pseudomonas fluorescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzaldehyde + pyruvate | - |
Pseudomonas fluorescens | trans-benzylidenepyruvate | - |
? | |
| benzaldehyde + pyruvate | - |
Pseudomonas fluorescens N3 | trans-benzylidenepyruvate | - |
? | |
| salicylaldehyde + pyruvate | - |
Pseudomonas fluorescens | trans-o-hydroxybenzylidenepyruvate | - |
? | |
| salicylaldehyde + pyruvate | - |
Pseudomonas fluorescens N3 | trans-o-hydroxybenzylidenepyruvate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 38000, His-tagged recombinant enzyme, SDS-PAGE | Pseudomonas fluorescens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| tHBP-HA | - |
Pseudomonas fluorescens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
at a fixed pH of 7.0 | Pseudomonas fluorescens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 50 | - |
Pseudomonas fluorescens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 40 | after 30 min incubation, the decreases of the activity are 64% at 40°C and 24% at 35°C, while at 30°C, the activity remains unchanged. However, at 30°C, the decreases of the activity are 24% after 5 h and 54% after 24 h | Pseudomonas fluorescens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Pseudomonas fluorescens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8.5 | - |
Pseudomonas fluorescens |
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