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Literature summary for 4.1.2.45 extracted from

  • Kuhm, A.E.; Knackmuss, H.J.; Stolz, A.
    Purification and properties of 2'-hydroxybenzalpyruvate aldolase from a bacterium that degrades naphthalenesulfonates (1993), J. Biol. Chem., 268, 9484-9489.
    View publication on PubMed
Search for more literature for 4.1.2.45

General Stability

General Stability Organism
-16°C, 4°C or 20°C, the enzyme is very stable in cell free extracts, after a 1-week incubation (protein concentration: 2.6 mg/ml) in Na/K-phosphate buffer (pH 7.3, 50 mM) the initial enzyme activity is completely recovered Sphingobium xenophagum

Inhibitors

Inhibitors Comment Organism Structure
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
-
 Sphingobium xenophagum
additional information no inhibition: benzylideneacetone, cinnamic acid Sphingobium xenophagum
p-chloromercuribenzoate enzyme is partially reactivated by addition of dithiothreitol Sphingobium xenophagum
Salicylaldehyde
-
 Sphingobium xenophagum
sodium borhydride
-
 Sphingobium xenophagum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
 (3E)-4-(2,6-dihydroxyphenyl)-2-oxobut-3-enoate pH 7.0 Sphingobium xenophagum
0.015
-
 (3E)-4-(2,4-dihydroxyphenyl)-2-oxobut-3-enoate pH 7.0 Sphingobium xenophagum
0.017
-
 (3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate pH 7.0 Sphingobium xenophagum
0.4
-
 benzylidenepyruvate pH 7.0 Sphingobium xenophagum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38500
-
 3 * 38500, SDS-PAGE Sphingobium xenophagum
120000
-
 gel filtration Sphingobium xenophagum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate + H2O Sphingobium xenophagum this reaction is part of the degradative pathways for naphthalene and naphthalenesulfonates by bacteria salicylaldehyde + pyruvate
-
 ?
(3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate + H2O Sphingobium xenophagum BN6 this reaction is part of the degradative pathways for naphthalene and naphthalenesulfonates by bacteria salicylaldehyde + pyruvate
-
 ?

Organism

Organism UniProt Comment Textmining
Sphingobium xenophagum
-
 BN6
-
Sphingobium xenophagum BN6
-
 BN6
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sphingobium xenophagum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
23.67
-
-
 Sphingobium xenophagum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3E)-4-(2,4-dihydroxyphenyl)-2-oxobut-3-enoate + H2O
-
 Sphingobium xenophagum 2,4-dihydroxybenzaldehyde + pyruvate
-
 ?
(3E)-4-(2,4-dihydroxyphenyl)-2-oxobut-3-enoate + H2O
-
 Sphingobium xenophagum BN6 2,4-dihydroxybenzaldehyde + pyruvate
-
 ?
(3E)-4-(2,6-dihydroxyphenyl)-2-oxobut-3-enoate + H2O
-
 Sphingobium xenophagum 2,6-dihydroxybenzaldehyde + pyruvate
-
 ?
(3E)-4-(2,6-dihydroxyphenyl)-2-oxobut-3-enoate + H2O
-
 Sphingobium xenophagum BN6 2,6-dihydroxybenzaldehyde + pyruvate
-
 ?
(3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate + H2O this reaction is part of the degradative pathways for naphthalene and naphthalenesulfonates by bacteria Sphingobium xenophagum salicylaldehyde + pyruvate
-
 ?
(3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate + H2O i.e. 2'-hydroxybenzylidinepyruvate, i.e. 2'-hydroxybenzalpyruvate, i.e. trans-O-hydroxybenzylidenepyruvate, intermediate formation of a stable Schiff base between enzyme and substrate Sphingobium xenophagum salicylaldehyde + pyruvate salicylaldehyde i.e. 2-hydroxybenzaldehyde ?
(3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate + H2O this reaction is part of the degradative pathways for naphthalene and naphthalenesulfonates by bacteria Sphingobium xenophagum BN6 salicylaldehyde + pyruvate
-
 ?
(3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate + H2O i.e. 2'-hydroxybenzylidinepyruvate, i.e. 2'-hydroxybenzalpyruvate, i.e. trans-O-hydroxybenzylidenepyruvate, intermediate formation of a stable Schiff base between enzyme and substrate Sphingobium xenophagum BN6 salicylaldehyde + pyruvate salicylaldehyde i.e. 2-hydroxybenzaldehyde ?
benzylidenepyruvate + H2O with 1% of the activity found with (E)-2'-hydroxybenzylidinepyruvate Sphingobium xenophagum benzaldehyde + pyruvate
-
 ?
additional information no activity with: benzylideneacetone and cinnamic acid Sphingobium xenophagum ?
-
 ?
additional information no activity with: benzylideneacetone and cinnamic acid Sphingobium xenophagum BN6 ?
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 38500, SDS-PAGE Sphingobium xenophagum

Synonyms

Synonyms Comment Organism
2'-hydroxybenzalpyruvate aldolase
-
 Sphingobium xenophagum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 in Tris/HCl or Na/K-phosphate buffer Sphingobium xenophagum

pH Range

pH Minimum pH Maximum Comment Organism
6 9 more than 80% of the maximal activity at pH 6.0 and pH 9.0, pH 5.0: 18% of maximal activity, pH 10.0: 14% of maximal activity Sphingobium xenophagum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.013
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate pH 7.0 Sphingobium xenophagum