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Literature summary for 4.1.2.43 extracted from
- Characterization of two recombinant 3-hexulose-6-phosphate synthases from the halotolerant obligate methanotroph Methylomicrobium alcaliphilum 20Z (2017), Biochemistry, 82, 176-185 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed as fusion protein with 6-phospho-3-hexulose isomerase in Escherichia coli (DE3) Rosetta cells | Methylotuvimicrobium alcaliphilum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | in the presence of 1 mM ADP, the enzyme retains 37% activity | Methylotuvimicrobium alcaliphilum |  |
| AMP | complete inhibition at 1 mM AMP | Methylotuvimicrobium alcaliphilum | |
| ATP | in the presence of 1 mM ATP, the enzyme retains 86% activity | Methylotuvimicrobium alcaliphilum | |
| additional information | not inhibited by diphosphate | Methylotuvimicrobium alcaliphilum | |
| phosphate | in the presence of 1 mM phosphate, the enzyme retains 95% activity | Methylotuvimicrobium alcaliphilum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
D-ribulose 5-phosphate | at pH 8.5 and 30°C | Methylotuvimicrobium alcaliphilum | |
| 0.98 | - |
formaldehyde | at pH 8.5 and 30°C | Methylotuvimicrobium alcaliphilum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | dependent on | Methylotuvimicrobium alcaliphilum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
gel filtration | Methylotuvimicrobium alcaliphilum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-ribulose 5-phosphate + formaldehyde | Methylotuvimicrobium alcaliphilum | - |
D-arabino-hex-3-ulose 6-phosphate | - |
? | |
| D-ribulose 5-phosphate + formaldehyde | Methylotuvimicrobium alcaliphilum 20Z | - |
D-arabino-hex-3-ulose 6-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methylotuvimicrobium alcaliphilum | - |
- |
- |
| Methylotuvimicrobium alcaliphilum 20Z | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Methylotuvimicrobium alcaliphilum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-ribulose 5-phosphate + formaldehyde | - |
Methylotuvimicrobium alcaliphilum | D-arabino-hex-3-ulose 6-phosphate | - |
? | |
| D-ribulose 5-phosphate + formaldehyde | - |
Methylotuvimicrobium alcaliphilum 20Z | D-arabino-hex-3-ulose 6-phosphate | - |
? | |
| additional information | the enzyme does not use D-glucose, D-glucose 6-phosphate, D-glucose 1-phosphate, D-fructose, D-fructose 6-phosphate, D-fructose 1,6-bisphosphate, D-ribose 5-phosphate, D-ribose 1-phosphate, or D-erythrose 4-phosphate as formaldehyde acceptors | Methylotuvimicrobium alcaliphilum | ? | - |
? | |
| additional information | the enzyme does not use D-glucose, D-glucose 6-phosphate, D-glucose 1-phosphate, D-fructose, D-fructose 6-phosphate, D-fructose 1,6-bisphosphate, D-ribose 5-phosphate, D-ribose 1-phosphate, or D-erythrose 4-phosphate as formaldehyde acceptors | Methylotuvimicrobium alcaliphilum 20Z | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 20000, SDS-PAGE | Methylotuvimicrobium alcaliphilum |
| homodimer | 2 * 22200, calculated from amino acid sequence | Methylotuvimicrobium alcaliphilum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HPS | - |
Methylotuvimicrobium alcaliphilum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Methylotuvimicrobium alcaliphilum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | the enzyme activity is 70% higher at 50°C than at 30°C | Methylotuvimicrobium alcaliphilum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 45.82 | - |
formaldehyde | at pH 8.5 and 30°C | Methylotuvimicrobium alcaliphilum | |
| 58.8 | - |
D-ribulose 5-phosphate | at pH 8.5 and 30°C | Methylotuvimicrobium alcaliphilum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
- |
Methylotuvimicrobium alcaliphilum |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 46.8 | - |
formaldehyde | at pH 8.5 and 30°C | Methylotuvimicrobium alcaliphilum | |
| 452.3 | - |
D-ribulose 5-phosphate | at pH 8.5 and 30°C | Methylotuvimicrobium alcaliphilum | |
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