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Literature summary for 4.1.2.43 extracted from

  • Orita, I.; Kita, A.; Yurimoto, H.; Kato, N.; Sakai, Y.; Miki, K.
    Crystal structure of 3-hexulose-6-phosphate synthase, a member of the orotidine 5-monophosphate decarboxylase suprafamily (2010), Proteins Struct. Funct. Bioinform., 78, 3488-3492.
    View publication on PubMed
Search for more literature for 4.1.2.43

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3) cells Mycobacterium gastri

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with a sulfate ion, sitting drop vapor diffusion method, using 18% (w/v) polyethylene glycol (PEG) 3350 and 0.2 M MgCl2 Mycobacterium gastri

Protein Variants

Protein Variants Comment Organism
H134A the mutation abolishes HPS activity Mycobacterium gastri
H134D the mutation abolishes HPS activity Mycobacterium gastri
H134K the mutation abolishes HPS activity Mycobacterium gastri
H134N the mutation abolishes HPS activity Mycobacterium gastri
H134S the mutation abolishes HPS activity Mycobacterium gastri
K61A the mutant exhibits 50-90% of the activity of the wild type enzyme Mycobacterium gastri
K61D the mutant exhibits 50-90% of the activity of the wild type enzyme Mycobacterium gastri
K61H the mutant exhibits 50-90% of the activity of the wild type enzyme Mycobacterium gastri
K61S the mutant exhibits 50-90% of the activity of the wild type enzyme Mycobacterium gastri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.49
-
 D-ribulose 5-phosphate pH and temperature not specified in the publication Mycobacterium gastri

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the enzyme requires an Mg2+-assisted formation of an enediolate intermediate for catalysis of its reaction. The active site of each HPS monomer contains a magnesium ion Mycobacterium gastri
sulfate the active site of each HPS monomer contains a sulfate ion Mycobacterium gastri

Organism

Organism UniProt Comment Textmining
Mycobacterium gastri Q9LBW4
-
-
Mycobacterium gastri MB19 Q9LBW4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mycobacterium gastri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribulose 5-phosphate + formaldehyde
-
 Mycobacterium gastri D-arabino-hex-3-ulose 6-phosphate
-
 ?
D-ribulose 5-phosphate + formaldehyde
-
 Mycobacterium gastri MB19 D-arabino-hex-3-ulose 6-phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer x-ray crystallography Mycobacterium gastri

Synonyms

Synonyms Comment Organism
HPS
-
 Mycobacterium gastri