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Literature summary for 4.1.2.43 extracted from
- 3-Hexulose-6-phosphate synthase from Acetobacter methanolicus MB58 (1990), Methods Enzymol., 188, 401-405.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 1 mM Cu2+ is inhibitory, and the effect is partly abolished by Mg2+ | Acidomonas methanolica |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM Ca2+ is less than 50% less effective in activity stimulation than 1 mM Mg2+ | Acidomonas methanolica | |
| Co2+ | 1 mM Co2+ is less than 50% less effective in activity stimulation than 1 mM Mg2+ | Acidomonas methanolica | |
| Fe2+ | 1 mM Fe2+ is less than 50% less effective in activity stimulation than 1 mM Mg2+ | Acidomonas methanolica | |
| Mg2+ | bivalent cations are essential for activity, most effective is Mg2+ | Acidomonas methanolica | |
| Mn2+ | 1 mM Mn2+ is 20% less effective in activity stimulation than 1 mM Mg2+ | Acidomonas methanolica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 20400 | - |
4 * 20400, SDS-PAGE | Acidomonas methanolica |
| 80000 | - |
gel filtration | Acidomonas methanolica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acidomonas methanolica | - |
- |
- |
| Acidomonas methanolica MB58 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate fractionation, DEAE-cellulose column chromatography, and Sephadex G-100 gel filtration | Acidomonas methanolica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.4 | - |
cell free extract, at 30°C | Acidomonas methanolica |
| 64 | - |
after 46fold purification, at 30°C | Acidomonas methanolica |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, purified enzyme, at least 1 year, no loss of activity | Acidomonas methanolica |
| 0°C to -4°C, purified enzyme in the presence of 3.2 M ammonium sulfate, at least 1 year, no loss of activity | Acidomonas methanolica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-ribulose 5-phosphate + formaldehyde | - |
Acidomonas methanolica | D-arabino-hex-3-ulose 6-phosphate | - |
r | |
| D-ribulose 5-phosphate + formaldehyde | - |
Acidomonas methanolica MB58 | D-arabino-hex-3-ulose 6-phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 20400, SDS-PAGE | Acidomonas methanolica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HPS | - |
Acidomonas methanolica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Acidomonas methanolica |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 60 | no decrease in activity is observed within 6 h at 40°C in the presence of 5 mM MgCl2, at 50°C and 60°C the enzyme is stable for 2.5 and 0.5 h, respectively, one-half the initial activities are found after 6 and 1.5 h respectively | Acidomonas methanolica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Acidomonas methanolica |
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Release 2023.1 (January 2023)
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