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Literature summary for 4.1.2.43 extracted from

  • Arfman, N.; Bystrykh, L.V.; Govorukhina, N.I.; Dijkhuizen, L.
    3-Hexulose-6-phosphate synthase from thermotolerant methylotroph Bacillus C1 (1990), Methods Enzymol., 188, 391-397.
    View publication on PubMed
Search for more literature for 4.1.2.43

Inhibitors

Inhibitors Comment Organism Structure
D-ribulose 5-phosphate commercial D-ribulose 5-phosphate inhibits the enzyme Bacillus sp. (in: Bacteria)
EDTA
-
 Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00147
-
 formaldehyde at 50°C Bacillus sp. (in: Bacteria)
0.0045
-
 D-ribulose 5-phosphate D-ribulose 5-phosphate generated from ribose 5-phosphate, at 50°C Bacillus sp. (in: Bacteria)
0.007
-
 D-ribulose 5-phosphate commercial D-ribulose 5-phosphate, at 50°C Bacillus sp. (in: Bacteria)

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 28% stimulation of activity at 1 mM Bacillus sp. (in: Bacteria)
Co2+ 100% stimulation of activity at 1 mM Bacillus sp. (in: Bacteria)
Cu2+ 33% stimulation of activity at 1 mM Bacillus sp. (in: Bacteria)
Mg2+ 98% stimulation of activity at 1 mM Bacillus sp. (in: Bacteria)
Mn2+ 100% stimulation of activity at 1 mM Bacillus sp. (in: Bacteria)
Ni2+ 97% stimulation of activity at 1 mM Bacillus sp. (in: Bacteria)
Zn2+ 39% stimulation of activity at 1 mM Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 SDS-PAGE Bacillus sp. (in: Bacteria)
32000
-
 gel filtration Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) C1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography, phenyl-Superose column chromatography, Superose 12 chromatography, and Sephadex G-25 gel filtration Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5
-
 cell free extract, at 50°C Bacillus sp. (in: Bacteria)
64
-
 after 18fold purification, at 50°C Bacillus sp. (in: Bacteria)

Storage Stability

Storage Stability Organism
-80°C, purified enzyme, in the presence of 5 mM MgSO4, 5 mM D-ribose 5-phosphate, and 1.75 units/ml phosphoriboisomerase, at least 5 months, no loss of activity Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribulose 5-phosphate + formaldehyde
-
 Bacillus sp. (in: Bacteria) D-arabino-hex-3-ulose 6-phosphate
-
 ?
D-ribulose 5-phosphate + formaldehyde
-
 Bacillus sp. (in: Bacteria) C1 D-arabino-hex-3-ulose 6-phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 27000, SDS-PAGE Bacillus sp. (in: Bacteria)

Synonyms

Synonyms Comment Organism
3-hexulose-6-phosphate synthase
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55 65 the enzyme retains more than 50% of its activity at 55°C after 1 h, the enzyme retains more than 50% of its activity at 65°C after 30 min Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Bacillus sp. (in: Bacteria)