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Literature summary for 4.1.2.43 extracted from
- 3-Hexulosephosphate synthase from Methylomonas aminofaciens 77a. Purification, properties and kinetics (1978), Biochim. Biophys. Acta, 523, 236-244.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 45% inhibition with 1 mM Cu2+ | Methylomonas aminofaciens |  |
| EDTA | the enzyme is completely inhibited in the presence of EDTA, but the activity is restored depending on the amount of MgC12 supplemented | Methylomonas aminofaciens | |
| Glutaraldehyde | 22% inhibition at 4 mM | Methylomonas aminofaciens | |
| glyceraldehyde | 13% inhibition at 4 mM | Methylomonas aminofaciens | |
| glycolaldehyde | 49% inhibition at 4 mM | Methylomonas aminofaciens | |
| glyoxylate | 12% inhibition at 4 mM | Methylomonas aminofaciens | |
| Hg2+ | 81% inhibition with 1 mM Hg2+ | Methylomonas aminofaciens | |
| methylglyoxal | 26% inhibition at 4 mM | Methylomonas aminofaciens | |
| Pb2+ | 48% inhibition with 1 mM Pb2+ | Methylomonas aminofaciens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.059 | - |
D-ribulose 5-phosphate | in 50 mM potassium phosphate buffer (pH 7.4), 5 mM MgCl2 | Methylomonas aminofaciens | |
| 0.29 | - |
formaldehyde | in 50 mM potassium phosphate buffer (pH 7.4), 5 mM MgCl2 | Methylomonas aminofaciens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | the enzyme activity is also promoted with 1 mM Cd2+ | Methylomonas aminofaciens | |
| Co2+ | the enzyme activity is also promoted with 5 mM Co2+ | Methylomonas aminofaciens | |
| Fe2+ | the enzyme activity is also promoted with 1 mM Fe2+ | Methylomonas aminofaciens | |
| Mg2+ | essential requirement for Mg2+ (5 mM) | Methylomonas aminofaciens | |
| Mn2+ | essential requirement for Mn2+ (5 mM) | Methylomonas aminofaciens | |
| Zn2+ | the enzyme activity is also promoted with 5 mM Zn2+ | Methylomonas aminofaciens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
2 * 23000, SDS-PAGE | Methylomonas aminofaciens |
| 45000 | - |
native enzyme, gel filtration | Methylomonas aminofaciens |
| 47000 | - |
sedimentation velocity | Methylomonas aminofaciens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methylomonas aminofaciens | - |
- |
- |
| Methylomonas aminofaciens 77a | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-cellulose column chromatography, hydroxylapatite column chromatography, Sephadex G-150 gel filtration, Sephadex G-100 gel filtration, and DEAE-Sephadex A-50 gel filtration | Methylomonas aminofaciens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.3 | - |
cell free extract | Methylomonas aminofaciens |
| 52 | - |
after 16fold purification | Methylomonas aminofaciens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-ribulose 5-phosphate + formaldehyde | the enzyme is specific for formaldehyde and D-ribulose 5-phosphate | Methylomonas aminofaciens | D-arabino-hex-3-ulose 6-phosphate | - |
? | |
| D-ribulose 5-phosphate + formaldehyde | the enzyme is specific for formaldehyde and D-ribulose 5-phosphate | Methylomonas aminofaciens 77a | D-arabino-hex-3-ulose 6-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 23000, SDS-PAGE | Methylomonas aminofaciens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3-hexulosephosphate synthase | - |
Methylomonas aminofaciens |
| D-arabino-3-hexulose 6-phosphate formaldehyde lyase | - |
Methylomonas aminofaciens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Methylomonas aminofaciens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 80 | almost full activity is retained after incubation at 40°C for 30 min, whereas at 80°C for 10 min the enzyme is completely inactivated | Methylomonas aminofaciens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Methylomonas aminofaciens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | stable in neutral to slightly alkaline solutions | Methylomonas aminofaciens |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Methylomonas aminofaciens | isoelectric focusing | - |
5.1 |
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