Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glycolic acid | glycolic acid inhibits the enzyme competitively with respect to formaldehyde | Mycobacterium gastri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
formaldehyde | apparent value, in 50 mM potassium phosphate, pH 7.5, with 5 mM MgCl2, at 30°C | Mycobacterium gastri | |
4.3 | - |
glycolaldehyde | apparent value, in 50 mM potassium phosphate, pH 7.5, with 5 mM MgCl2, at 30°C | Mycobacterium gastri | |
5.7 | - |
methylglyoxal | apparent value, in 50 mM potassium phosphate, pH 7.5, with 5 mM MgCl2, at 30°C | Mycobacterium gastri |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 1 mM Mg2+ is absolutely required for the enzyme activity and stability | Mycobacterium gastri | |
Mn2+ | Mg2+ can be replaced by Mn2+ (1 mM) | Mycobacterium gastri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
SDS-PAGE | Mycobacterium gastri |
43000 | - |
native enzyme, gel filtration | Mycobacterium gastri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium gastri | - |
- |
- |
Mycobacterium gastri MB19 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
phenyl-Sepharose column chromatography and DEAE-Sephacel column chromatography | Mycobacterium gastri |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.2 | - |
cell free extract, at 30°C | Mycobacterium gastri |
74.2 | - |
after 23fold purification, at 30°C | Mycobacterium gastri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 5-phosphate + formaldehyde | - |
Mycobacterium gastri | D-arabino-hex-3-ulose 6-phosphate | - |
? | |
D-ribulose 5-phosphate + formaldehyde | - |
Mycobacterium gastri MB19 | D-arabino-hex-3-ulose 6-phosphate | - |
? | |
D-ribulose 5-phosphate + glycolaldehyde | - |
Mycobacterium gastri | 4-heptulose 7-phosphate | - |
? | |
D-ribulose 5-phosphate + glycolaldehyde | - |
Mycobacterium gastri MB19 | 4-heptulose 7-phosphate | - |
? | |
D-ribulose 5-phosphate + methylglyoxal | - |
Mycobacterium gastri | ? | - |
? | |
D-ribulose 5-phosphate + methylglyoxal | - |
Mycobacterium gastri MB19 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-hexulose phosphate synthase | - |
Mycobacterium gastri |
HPS | - |
Mycobacterium gastri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Mycobacterium gastri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Mycobacterium gastri |