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Literature summary for 4.1.2.43 extracted from

  • Kato, N.; Miyamoto, N.; Shimao, M.; Sakazawa, C.
    3-Hexulose phosphate synthase from a new facultative methylotroph, Mycobacterium gastri MB19 (1988), Agric. Biol. Chem., 52, 2659-2661.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
glycolic acid glycolic acid inhibits the enzyme competitively with respect to formaldehyde Mycobacterium gastri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4
-
formaldehyde apparent value, in 50 mM potassium phosphate, pH 7.5, with 5 mM MgCl2, at 30°C Mycobacterium gastri
4.3
-
glycolaldehyde apparent value, in 50 mM potassium phosphate, pH 7.5, with 5 mM MgCl2, at 30°C Mycobacterium gastri
5.7
-
methylglyoxal apparent value, in 50 mM potassium phosphate, pH 7.5, with 5 mM MgCl2, at 30°C Mycobacterium gastri

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 1 mM Mg2+ is absolutely required for the enzyme activity and stability Mycobacterium gastri
Mn2+ Mg2+ can be replaced by Mn2+ (1 mM) Mycobacterium gastri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
SDS-PAGE Mycobacterium gastri
43000
-
native enzyme, gel filtration Mycobacterium gastri

Organism

Organism UniProt Comment Textmining
Mycobacterium gastri
-
-
-
Mycobacterium gastri MB19
-
-
-

Purification (Commentary)

Purification (Comment) Organism
phenyl-Sepharose column chromatography and DEAE-Sephacel column chromatography Mycobacterium gastri

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2
-
cell free extract, at 30°C Mycobacterium gastri
74.2
-
after 23fold purification, at 30°C Mycobacterium gastri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribulose 5-phosphate + formaldehyde
-
Mycobacterium gastri D-arabino-hex-3-ulose 6-phosphate
-
?
D-ribulose 5-phosphate + formaldehyde
-
Mycobacterium gastri MB19 D-arabino-hex-3-ulose 6-phosphate
-
?
D-ribulose 5-phosphate + glycolaldehyde
-
Mycobacterium gastri 4-heptulose 7-phosphate
-
?
D-ribulose 5-phosphate + glycolaldehyde
-
Mycobacterium gastri MB19 4-heptulose 7-phosphate
-
?
D-ribulose 5-phosphate + methylglyoxal
-
Mycobacterium gastri ?
-
?
D-ribulose 5-phosphate + methylglyoxal
-
Mycobacterium gastri MB19 ?
-
?

Synonyms

Synonyms Comment Organism
3-hexulose phosphate synthase
-
Mycobacterium gastri
HPS
-
Mycobacterium gastri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Mycobacterium gastri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
Mycobacterium gastri