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Literature summary for 4.1.2.42 extracted from
- Expanding the threonine aldolase toolbox for the asymmetric synthesis of tertiary alpha-amino acids (2015), Appl. Microbiol. Biotechnol., 99, 9651-9661 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Pseudomonas sp. |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.5 | - |
D-beta-phenylserine | 25°C, pH 8.0 | Pseudomonas sp. |  |
| 6.2 | - |
alpha-methyl-beta-phenylserine | 25°C, pH 8.0 | Pseudomonas sp. | |
| 15.9 | - |
D-threonine | pH 8.0, temperature not specified in the publication | Pseudomonas sp. | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas sp. | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-methyl-beta-phenylserine | - |
Pseudomonas sp. | D-alanine + benzaldehyde | - |
? | |
| D-alanine + 3-nitrobenzaldehyde | - |
Pseudomonas sp. | beta-(3-nitrophenyl)-alpha-methyl-serine | - |
? | |
| D-alanine + benzaldehyde | - |
Pseudomonas sp. | 2-amino-3-hydroxy-2-methyl-3-phenylpropanoic acid | - |
? | |
| D-alanine + hexanal | - |
Pseudomonas sp. | 2-amino-3-hydroxy-2-methyloctanoate | - |
? | |
| D-beta-phenylserine | - |
Pseudomonas sp. | glycine + benzaldehyde | - |
? | |
| D-serine + 3-nitrobenzaldehyde | - |
Pseudomonas sp. | beta-(3-nitrophenyl)-alpha-hydroxymethyl-serine | - |
? | |
| D-serine + benzaldehyde | - |
Pseudomonas sp. | 2-amino-3-hydroxy-2-hydroxymethyl-3-phenylpropanoic acid | - |
? | |
| D-threonine | - |
Pseudomonas sp. | glycine + acetaldehyde | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DTA | - |
Pseudomonas sp. |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.033 | - |
alpha-methyl-beta-phenylserine | 25°C, pH 8.0 | Pseudomonas sp. | |
| 0.4 | - |
D-threonine | pH 8.0, temperature not specified in the publication | Pseudomonas sp. | |
| 1.4 | - |
D-beta-phenylserine | 25°C, pH 8.0 | Pseudomonas sp. | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | pyridoxal 5'-phosphate-dependent enzyme | Pseudomonas sp. | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00533 | - |
alpha-methyl-beta-phenylserine | 25°C, pH 8.0 | Pseudomonas sp. | |
| 0.025 | - |
D-threonine | pH 8.0, temperature not specified in the publication | Pseudomonas sp. | |
| 0.91 | - |
D-beta-phenylserine | 25°C, pH 8.0 | Pseudomonas sp. | |
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