Cloned (Comment) | Organism |
---|---|
cloned in Escherichia coli | Achromobacter xylosoxidans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
MnCl2 | - |
Achromobacter xylosoxidans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Achromobacter xylosoxidans | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-bromobenzaldehyde + glycine | analytical yield: 6%, de: 35%, conditions: 1 ml solution containing glycine (1 M), benzaldehyde (or its derivate) (100 mM), pyridoxal 5'-phosphate (50 mM), MnCl2 (50 mM), and D-threonine aldolase (23 U) at 5°C, 4 h, ee >99% (D) for all reactions | Achromobacter xylosoxidans | D-2-bromophenylserine | - |
? | |
2-chlorobenzaldehyde + glycine | analytical yield: 27%, de: 67%, conditions: 1 ml solution containing glycine (1 M), benzaldehyde (or its derivate) (100 mM), pyridoxal 5'-phosphate (50 mM), MnCl2 (50 mM), and D-threonine aldolase (23 U) at 5°C, 4 h, ee >99% (D) for all reactions | Achromobacter xylosoxidans | D-2-chlorophenylserine | - |
? | |
2-fluorobenzaldehyde + glycine | analytical yield: 68%, de: 95%, conditions: 1 ml solution containing glycine (1 M), benzaldehyde (or its derivate) (100 mM), pyridoxal 5'-phosphate (50 mM), MnCl2 (50 mM), and D-threonine aldolase (23 U) at 5°C, 4 h, ee >99% (D) for all reactions | Achromobacter xylosoxidans | D-2-fluorophenylserine | - |
? | |
2-nitrobenzaldehyde + glycine | analytical yield: 18%, de: 65%, conditions: 1 ml solution containing glycine (1 M), benzaldehyde (or its derivate) (100 mM), pyridoxal 5'-phosphate (50 mM), MnCl2 (50 mM), and D-threonine aldolase (23 U) at 5°C, 4 h, ee >99% (D) for all reactions | Achromobacter xylosoxidans | D-2-nitrophenylserine | - |
? | |
3,4-methylenedioxybenzaldehyde + glycine | analytical yield: 16%, de: 46%, conditions: 1 ml solution containing glycine (1 M), benzaldehyde (or its derivate) (100 mM), pyridoxal 5'-phosphate (50 mM), MnCl2 (50 mM), and D-threonine aldolase (23 U) at 5°C, 4 h, ee >99% (D) for all reactions | Achromobacter xylosoxidans | D-3,4-methylenedioxyphenylserine | - |
? | |
3-bromobenzaldehyde + glycine | analytical yield: 43%, de: 71%, conditions: 1 ml solution containing glycine (1 M), benzaldehyde (or its derivate) (100 mM), pyridoxal 5'-phosphate (50 mM), MnCl2 (50 mM), and D-threonine aldolase (23 U) at 5°C, 4 h, ee >99% (D) for all reactions | Achromobacter xylosoxidans | D-3-bromophenylserine | - |
? | |
3-chlorobenzaldehyde + glycine | analytical yield: 60%, de: 85%, conditions: 1 ml solution containing glycine (1 M), benzaldehyde (or its derivate) (100 mM), pyridoxal 5'-phosphate (50 mM), MnCl2 (50 mM), and D-threonine aldolase (23 U) at 5°C, 4 h, ee >99% (D) for all reactions | Achromobacter xylosoxidans | D-3-chlorophenylserine | - |
? | |
3-fluorobenzaldehyde + glycine | analytical yield: 54%, de: 81%, conditions: 1 ml solution containing glycine (1 M), benzaldehyde (or its derivate) (100 mM), pyridoxal 5'-phosphate (50 mM), MnCl2 (50 mM), and D-threonine aldolase (23 U) at 5°C, 4 h, ee >99% (D) for all reactions | Achromobacter xylosoxidans | D-3-fluorophenylserine | - |
? | |
3-hydroxybenzaldehyde + glycine | analytical yield: 76%, de: 86%, conditions: 1 ml solution containing glycine (1 M), benzaldehyde (or its derivate) (100 mM), pyridoxal 5'-phosphate (50 mM), MnCl2 (50 mM), and D-threonine aldolase (23 U) at 5°C, 4 h, ee >99% (D) for all reactions | Achromobacter xylosoxidans | D-3-hydroxyphenylserine | - |
? | |
3-nitrobenzaldehyde + glycine | analytical yield: 90%, de: 80%, conditions: 1 ml solution containing glycine (1 M), benzaldehyde (or its derivate) (100 mM), pyridoxal 5'-phosphate (50 mM), MnCl2 (50 mM), and D-threonine aldolase (23 U) at 5°C, 4 h, ee >99% (D) for all reactions | Achromobacter xylosoxidans | D-3-nitrophenylserine | - |
? | |
4-(methylsulfonyl)benzaldehyde + glycine | analytical yield: 63%, de: 99%, conditions: 1 ml solution containing glycine (1 M), benzaldehyde (or its derivate) (100 mM), pyridoxal 5'-phosphate (50 mM), MnCl2 (50 mM), and D-threonine aldolase (23 U) at 5°C, 4 h, ee >99% (D) for all reactions | Achromobacter xylosoxidans | D-3-(4-methylsulfonylphenyl)serine | - |
? | |
4-bromobenzaldehyde + glycine | analytical yield: 12%, de: 74%, conditions: 1 ml solution containing glycine (1 M), benzaldehyde (or its derivate) (100 mM), pyridoxal 5'-phosphate (50 mM), MnCl2 (50 mM), and D-threonine aldolase (23 U) at 5°C, 4 h, ee >99% (D) for all reactions | Achromobacter xylosoxidans | D-4-bromophenylserine | - |
? | |
4-chlorobenzaldehyde + glycine | analytical yield: 26%, de: 86%, conditions: 1 ml solution containing glycine (1 M), benzaldehyde (or its derivate) (100 mM), pyridoxal 5'-phosphate (50 mM), MnCl2 (50 mM), and D-threonine aldolase (23 U) at 5°C, 4 h, ee >99% (D) for all reactions | Achromobacter xylosoxidans | D-4-chlorophenylserine | - |
? | |
4-fluorobenzaldehyde + glycine | analytical yield: 42%, de: 91%, conditions: 1 ml solution containing glycine (1 M), benzaldehyde (or its derivate) (100 mM), pyridoxal 5'-phosphate (50 mM), MnCl2 (50 mM), and D-threonine aldolase (23 U) at 5°C, 4 h, ee >99% (D) for all reactions | Achromobacter xylosoxidans | D-4-fluorophenylserine | - |
? | |
4-formylbenzenesulfonamide + glycine | analytical yield: 53%, de: above 90%, conditions: 1 ml solution containing glycine (1 M), benzaldehyde (or its derivate) (100 mM), pyridoxal 5'-phosphate (50 mM), MnCl2 (50 mM), and D-threonine aldolase (23 U) at 5°C, 4 h, ee >99% (D) for all reactions | Achromobacter xylosoxidans | D-2-amino-3-hydroxy-3-(4-sulfamoylphenyl)-propanoic acid | - |
? | |
4-hydroxybenzaldehyde + glycine | analytical yield: 15%, de: 70%, conditions: 1 ml solution containing glycine (1 M), benzaldehyde (or its derivate) (100 mM), pyridoxal 5'-phosphate (50 mM), MnCl2 (50 mM), and D-threonine aldolase (23 U) at 5°C, 4 h, ee >99% (D) for all reactions | Achromobacter xylosoxidans | D-4-hydroxyphenylserine | - |
? | |
4-nitrobenzaldehyde + glycine | analytical yield: 31%, de: 75%, conditions: 1 ml solution containing glycine (1 M), benzaldehyde (or its derivate) (100 mM), pyridoxal 5'-phosphate (50 mM), MnCl2 (50 mM), and D-threonine aldolase (23 U) at 5°C, 4 h, ee >99% (D) for all reactions | Achromobacter xylosoxidans | D-4-nitrophenylserine | - |
? | |
benzaldehyde + glycine | analytical yield: 79%, de: 98%, conditions: 1 ml solution containing glycine (1 M), benzaldehyde (or its derivate) (100 mM), pyridoxal 5'-phosphate (50 mM), MnCl2 (50 mM), and D-threonine aldolase (23 U) at 5°C, 4 h, ee >99% (D) for all reactions | Achromobacter xylosoxidans | D-phenylserine | - |
? | |
additional information | a high excess of glycine shifts the equilibrium toward the phenylserine side and thus 10 equiv of glycine is utilized (1 M glycine, 100 mM benzaldehyde) throughout the entire study | Achromobacter xylosoxidans | ? | - |
? | |
additional information | in a systematic study, 21 ring-substituted benzaldehydes are reacted with glycine under catalysis with a L-threonine aldolase (LTA) from Pseudomonas putida and a D-threonine aldolase (DTA) from Alcaligenes xylosoxidans to form the corresponding beta-hydroxy-alpha-amino acids | Achromobacter xylosoxidans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
D-threonine aldolase | - |
Achromobacter xylosoxidans |
DTA | - |
Achromobacter xylosoxidans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | - |
- |
Achromobacter xylosoxidans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
for the synthesis of D-phenylserine employing D-threonine aldolase, the rate of the aldol reaction is studied from 5 to 40°C. Lowering the temperature to 5°C results in an increase of diastereoselectivity (up to 98%) as well as in a higher analytical yield (79%) after 4 h | Achromobacter xylosoxidans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Achromobacter xylosoxidans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.5 | D-threonine aldolase shows little activity at pH 6.0. However, by increasing the pH to 9.5 the analytical yield of D-phenylserine is doubled retaining high diastereoselectivity as compared to pH 8.0 | Achromobacter xylosoxidans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Achromobacter xylosoxidans |