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Literature summary for 4.1.2.4 extracted from

  • Kim, Y.M.; Chang, Y.H.; Choi, N.S.; Kim, Y.; Song, J.J.; Kim, J.S.
    Cloning, expression, and characterization of a new deoxyribose 5-phosphate aldolase from Yersinia sp. EA015 (2009), Protein Expr. Purif., 68, 196-200.
    View publication on PubMed

Application

Application Comment Organism
biotechnology DERA has the potential to resist high concentrations of acetaldehyde and may serve as an industrial catalyst Yersinia sp. EA015

Cloned(Commentary)

Cloned (Comment) Organism
into a pET302/NT-his expression vector and overexpressed in Escherichia coli BL21 (DE3) Yersinia sp. EA015

Inhibitors

Inhibitors Comment Organism Structure
additional information 2-deoxy-D-ribose-5-phosphate-producing activity of the enzyme remains even at 300 mM acetaldehyde Yersinia sp. EA015

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
9.1
-
2-deoxy-D-ribose-5-phosphate in 100 mM Tris-HCl buffer, pH 8.8, 0.3 mM NADH, 10 U alcohol dehydrogenase, 1 mM 2-deoxy-D-ribose-5-phosphate Yersinia sp. EA015

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24800
-
x * 24800, SDS-PAGE Yersinia sp. EA015

Organism

Organism UniProt Comment Textmining
Yersinia sp. EA015 C0LSK9
-
-

Purification (Commentary)

Purification (Comment) Organism
by Ni-NTA affinity chromatography, 2.4fold, with a yield of 43% Yersinia sp. EA015

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
56
-
crude extract Yersinia sp. EA015
137
-
2.4fold purified enzyme Yersinia sp. EA015

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-ribose-5-phosphate
-
Yersinia sp. EA015 D-glyceraldehyde 3-phosphate + acetaldehyde
-
?

Subunits

Subunits Comment Organism
? x * 24800, SDS-PAGE Yersinia sp. EA015

Synonyms

Synonyms Comment Organism
deoxyribose 5-phosphate aldolase
-
Yersinia sp. EA015
DERA
-
Yersinia sp. EA015

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Yersinia sp. EA015

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Yersinia sp. EA015