Application | Comment | Organism |
---|---|---|
biotechnology | DERA has the potential to resist high concentrations of acetaldehyde and may serve as an industrial catalyst | Yersinia sp. EA015 |
Cloned (Comment) | Organism |
---|---|
into a pET302/NT-his expression vector and overexpressed in Escherichia coli BL21 (DE3) | Yersinia sp. EA015 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | 2-deoxy-D-ribose-5-phosphate-producing activity of the enzyme remains even at 300 mM acetaldehyde | Yersinia sp. EA015 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.1 | - |
2-deoxy-D-ribose-5-phosphate | in 100 mM Tris-HCl buffer, pH 8.8, 0.3 mM NADH, 10 U alcohol dehydrogenase, 1 mM 2-deoxy-D-ribose-5-phosphate | Yersinia sp. EA015 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24800 | - |
x * 24800, SDS-PAGE | Yersinia sp. EA015 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Yersinia sp. EA015 | C0LSK9 | - |
- |
Purification (Comment) | Organism |
---|---|
by Ni-NTA affinity chromatography, 2.4fold, with a yield of 43% | Yersinia sp. EA015 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
56 | - |
crude extract | Yersinia sp. EA015 |
137 | - |
2.4fold purified enzyme | Yersinia sp. EA015 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-D-ribose-5-phosphate | - |
Yersinia sp. EA015 | D-glyceraldehyde 3-phosphate + acetaldehyde | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 24800, SDS-PAGE | Yersinia sp. EA015 |
Synonyms | Comment | Organism |
---|---|---|
deoxyribose 5-phosphate aldolase | - |
Yersinia sp. EA015 |
DERA | - |
Yersinia sp. EA015 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Yersinia sp. EA015 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Yersinia sp. EA015 |