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Literature summary for 4.1.2.4 extracted from
- The first crystal structure of archaeal aldolase. Unique tetrameric structure of 2-deoxy-D-ribose-5-phosphate aldolase from the hyperthermophilic archaea Aeropyrum pernix (2003), J. Biol. Chem., 278, 10799-10806.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressen in Escherichia coli | Aeropyrum pernix |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapor diffusion method | Aeropyrum pernix |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.057 | - |
2-deoxy-D-ribose 5-phosphate | 50°C, pH 6.5 | Aeropyrum pernix |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
4 * 24000, SDS-PAGE | Aeropyrum pernix |
| 93000 | - |
gel-filtration | Aeropyrum pernix |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Ethanol | stable up to 40% for 30 min at 50°C | Aeropyrum pernix |
| Methanol | stable up to 50% for 30 min at 50°C | Aeropyrum pernix |
| N,N-dimethylformamide | stable up to 50% for 30 min at 50°C | Aeropyrum pernix |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aeropyrum pernix | Q9Y948 | K1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aeropyrum pernix |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
50°C | Aeropyrum pernix |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-deoxy-D-ribose 5-phosphate | - |
Aeropyrum pernix | D-glyceraldehyde 3-phosphate + acetaldehyde | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 24000, SDS-PAGE | Aeropyrum pernix |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
assay at | Aeropyrum pernix |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
no loss of activity after 60 min | Aeropyrum pernix |
| 100 | - |
no loss of activity after 10 min | Aeropyrum pernix |
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