Literature summary for 4.1.2.38 extracted from

Van Den Wittenboer, A.; Niemeijer, B.; Karmee, S.; Ansorge-Schumacher, M.
Systematic assessment of the stability of benzaldehyde lyase in aqueous-organic biphasic systems and its stabilization by modification with methoxy-poly(ethylene) glycol (2010), J. Mol. Catal. B, 67, 208-213.

No PubMed abstract available

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli SG13009 pRep4 cells	Pseudomonas fluorescens

General Stability

General Stability	Organism
the half-life of BAL in the presence of the investigated solvents, but without formation of distinct solvent phases is 17.3 h, 5.6 h and 3.2 h for saturation with methyl-tert-butylether, 2-octanone, and toluene, respectively, in non-reactive monophasic media. With MTBE, 2-octanone, and toluene, very similar half-lives of 1.5 h, 1.4 h and 2.0 h are found for unmodified BAL under process conditions in reactive biphasic media. The initial activity of BAL decreases to approximately 60%, when methoxy-poly(ethylene) glycol (mPEG)750 is attached, and to 70%, when a longer-chain mPEG2000 is used. Overall, the modification with mPEG leads to good stabilization of BAL under non-reactive conditions. The strongest effect is achieved against toluene, where the stability of mPEG2000-BAL surpasses the stability of unmodified BAL by factor 18	Pseudomonas fluorescens

Organism

the half-life of BAL in the presence of the investigated solvents, but without formation of distinct solvent phases is 17.3 h, 5.6 h and 3.2 h for saturation with methyl-tert-butylether, 2-octanone, and toluene, respectively, in non-reactive monophasic media. With MTBE, 2-octanone, and toluene, very similar half-lives of 1.5 h, 1.4 h and 2.0 h are found for unmodified BAL under process conditions in reactive biphasic media. The initial activity of BAL decreases to approximately 60%, when methoxy-poly(ethylene) glycol (mPEG)750 is attached, and to 70%, when a longer-chain mPEG2000 is used. Overall, the modification with mPEG leads to good stabilization of BAL under non-reactive conditions. The strongest effect is achieved against toluene, where the stability of mPEG2000-BAL surpasses the stability of unmodified BAL by factor 18

Pseudomonas fluorescens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Pseudomonas fluorescens

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas fluorescens	-	biovar I	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-Sepharose column chromatography	Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-furaldehyde + 2-furaldehyde	-	Pseudomonas fluorescens	2,2'-furoin	-	r
3,5-dimethoxybenzaldehyde + 3,5-dimethoxybenzaldehyde	-	Pseudomonas fluorescens	3,3',5,5'-tetramethoxybenzoin	-	r

Synonyms

Synonyms	Comment	Organism
BAL	-	Pseudomonas fluorescens
benzaldehyde lyase	-	Pseudomonas fluorescens

Cofactor

Cofactor	Comment	Organism	Structure
thiamine diphosphate	-	Pseudomonas fluorescens