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Literature summary for 4.1.2.38 extracted from

  • Mikolajek, R.J.; Spiess, A.C.; Pohl, M.; Buechs, J.
    Propioin synthesis using thiamine diphosphate-dependent enzymes (2009), Biotechnol. Prog., 25, 132-138.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
propanal deactivates in the presence of high concentration Pseudomonas fluorescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
415
-
propanal in aqueous solution Pseudomonas fluorescens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Pseudomonas fluorescens

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
biovar I
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.09
-
propanal carboligation Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
propanal + propanal
-
Pseudomonas fluorescens (S)-propioin
-
?

Synonyms

Synonyms Comment Organism
BAL
-
Pseudomonas fluorescens
benzaldehyde lyase
-
Pseudomonas fluorescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
propioin reaction Pseudomonas fluorescens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 40 at 30 and 40°C in aqueous solution, the relative activity increases slightly during the first measurements, and after that the decrease is very low. At 50°C, a strong exponential decrease is observed. No significant differences of half-life between 30°C and 40°C with 16 and 18 h, respectively, whereas at 50°C, a strong decrease of the half-life is observed with 0.4 h Pseudomonas fluorescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Pseudomonas fluorescens

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate
-
Pseudomonas fluorescens